黄芪对苍耳子肝毒性影响的实验研究

目的:观察黄芪对苍耳子肝毒性的影响。方法:65只雄性昆明小鼠分为5组(每组13只):1:1组(苍耳子5g/kg加黄芪5g/kg)、1:2组(苍耳子5g/kg加黄芪10g/kg)、2:1组(苍耳子5g/kg加黄芪2.5g/kg)、苍耳子组(苍耳子5g/kg)及对照组(生理盐水)。连续给药4周,每周测1次体重,末次给药后检测血清AST、ALT水平,计算肝脏指数,测定肝脏谷胱甘肽过氧化物酶(GSH-PX)、还原型谷胱甘肽(GST)和丙二醛(MDA)。结果:1:1组和2:1组小鼠体重增加量明显高于苍耳子组(P<0.05),肝脏指数低于苍耳子组(P<0.05)。各实验组小鼠血清AST、ALT水平均低于苍耳子组,其中2:1组与苍耳子组比较差异有统计学意义(P<0.05);1:2组和2:1组GSH-PX、GST活力均高于苍耳子组,差异均有统计学意义(P<0.05;P<0.01);1:2与2:1组MDA含量均低于苍耳子组,其中2:1组与苍耳子组比较差异有统计学意义(P<0.05)。结论:配伍不同比例的黄芪可在一定程度上降低苍耳子的肝脏毒性作用,在3组中,以2:1组的效果较好。

Experimental studies of effects of milkvetch root (Radix Astragali) on hepatotoxicity induced by cocklebur fruit (Fructus Xanthii)

Objective: To observe the effects of milkvetch root (Radix Astragali) on hepatotoxicity induced by cocklebur fruit (Fructus Xanthii). Methods: Sixty-five male mice were divided into following five groups (13 mice in each group): 1:1 group (cocklebur fruit 5 g/kg plus milkvetch root 5 g/kg), 1:2 group (cocklebur fruit 5 g/kg plus milkvetch root 10 g/kg), 2:1 group (cocklebur fruit 5 g/kg plus milkvetch root 2.5 g/kg), cocklebur fruit group (cocklebur fruit 5 g/kg), and control group (physiological saline). The duration of administration was 4 weeks, and the weight of mice was measured weekly. After the last administration of the medicines, the serum levels of ALT and AST were measured, the liver index was calculated, and the GSH-PX, GST, and MDA of the liver were measured. Results: The weight gain in the 1:1 groups and 2:1 groups was marked higher than that in the cocklebur fruit group (P< 0.05), the liver index was lower than that in the cocklebur fruit group (P<0.05). The serum levels of AST and ALT in the three experimental groups were lower than those in the cocklebur fruit group, and there were statistically differences in comparison of the 2:1 group with the cocklebur fruit group. The activity of GSH-PX and GST in the 1: 2 group and 2:1 group were higher than those in the cocklebur fruit group, the differences were statistically significant (P<0.05; P<0.01). The content of MDA in the 1:2 group and 2:1 group were lower than that in the cocklebur fruit group, there were stastistically differences in comparison of the 2:1 group with the cocklebur fruit group (P<0.05). Conclusion: Milkvetch root could decrease the hepatoxicity of cocklebur fruit, and the 2:1 groupis better than other ones.