细菌L型的医院感染及其质粒谱分析研究

目的为了全面防止医院感染，我们对细菌Ｌ型的医院感染进行了研究。方法对医院内环境中８００份标本及医院感染患者９１８份标本分离出的金黄色葡萄球菌Ｌ型、大肠埃希氏菌Ｌ型及铜绿假单胞菌Ｌ型进行了质粒谱分析和药敏试验。结果本院环境及医院感染患者细菌Ｌ型总检出率分别为１９．０％和１６．７％。不同来源的３种细菌Ｌ型的多重耐药性无显著性差异。不同来源３种细菌Ｌ型的同源性比例均较高，分别为９０．３％、７５．０％、８４．２％。结论细菌Ｌ型医院感染患者的病原菌主要来自医院内环境

Study on the L form Bacteria Nosocomial Infection with Plasmid Profile Analysis

OBJECTIVE In order to prevent nosocomial infection, the L form bacteria were studied. METHODS 800 samples carried out from the hospital envionment and 918 samples from the nosocomial infected patients. Three strains of L forms (Staphyoeocaus aureus L form, Eschherichia colo L from, Pandomonos L form). were isolated and identified. RESULTS The general culture positive rates of 3 strains of L form bacteria of the hospital environment and the nosocomial patients were 19.0% and 16.7% respectiveoy. The multiple antbiotic resistance tests of the three strains from two different origins were of no significante difference (P>0.01). The homology ratios of the three strains of L form bacteria from two different origins were ruther high ie, 90.3%, 75.0% and 84.2% respectively. CONCLUSIONS The pathogenic organisms of the nosocomial infected patients by L form bacteria may be contamincded from the hospital environment.