壬基酚对大鼠F1子代脑基因表达的影响

目的:研究壬基酚通过妊娠期暴露是否会影响F1子代的脑发育。方法:对SD孕鼠染毒NP,从妊娠第7天开始持续到产后第二天,分别提取NP组和对照组F1代2d龄大鼠的脑组织mRNA,表达谱基因芯片检测脑基因的表达。用Q-PCR法验证F1雄性子代大脑泛素特异性蛋白酶8、ATP结合盒转运子和内磺肽蛋白这三个下调基因的表达,然后用Western blotting法检测F1子代大脑泛素特异性蛋白酶8的蛋白表达。结果:ATP结合盒转运子(ATP-binding cassette,ABCA)、内璜肽蛋白(Endosulfine alph,Ensa)、泛素特异性蛋白酶8(Ubiquitin Specific Protease 8,USP8/UBPy)基因和蛋白表达下调。结论:NP可影响神经细胞的正常结构和信号转导功能,并提示可以干扰机体内分泌系统、脂质和能量代谢及一系列正常生理功能。

Effects of nonylphenol on brain gene expression profiles of F1 generation

Objective：To investigate the adverse effects of nonylphenol （NP） on the development of brain of the F1 male rats after gestation exposure.Methods： Pregnant rats were exposed to NP at doses of 0 and 200mg/kg body weights, respectively from pregnant days 7 to after pregnancy to 2 days post partum. The 2 days brain tissue of the F1 progeny of male rats were tak- en, mRNA were extracted. The RQ-PCR method and Western blotting were used to detect the expression of ubiquitin-specific pro- tease 8 protein and AW141129, BE096678. NM-021842 abnormal gene expression, and analysis of possible mechanisms.Re- suits： ATP-binding cassette transporter （ATP-binding cassette,ABCA）, a-Endosulfine protein （Endosulfine alpha,Ensa） , ubiqui- tin-specific protease 8 （USP8 / UBPy） gene and protein expression decreased;.Conclusion：Nonylphenol can effect the structure of neurocyte and function of signal transduction.NP can interfere with neuroendocrine system function, affect lipid and energy metab- olism and a series of normal physiological function.