治咳川贝枇杷滴丸祛痰镇咳药效学评价及对肺组织Muc5AC表达的影响

目的 研究治咳川贝枇杷滴丸（CBPP）的祛痰、镇咳作用，以及对肺组织Muc5AC表达的影响，明确其药效特点，为中药临床定位提供实验依据。方法 采用雄性SPF级ICR小鼠，分别为对照（生理盐水）组、盐酸氨溴索口服液（阳性药，临床等效剂量11.7 mg/kg）组和CBPP低、中、高剂量（70.2、140.4、280.8 mg/kg）组，采用气管酚红排泌实验结合分光光度计法探究CBPP的祛痰药效；利用氨水引咳实验观察CBPP高剂量组的镇咳作用；采用实时荧光定量PCR（qRT-PCR）法检测CBPP高剂量组对肺组织中Muc5AC mRNA表达的影响。家兔随机分为对照组、盐酸氨溴索口服液（10.5 mg/mL）组、CBPP（252 mg/mL）组，利用离体气管纤毛运动实验考察CBPP对气管纤毛运动的影响。结果 酚红排泌实验显示，与对照组比较，阳性药和CBPP显著促进正常小鼠气管酚红排泌量增加（P<0.05、0.01），且CBPP作用呈剂量相关性；家兔离体气管纤毛运动实验显示，与对照组比较，阳性药和CBPP显著促进纤毛运动（P<0.05），进而促进痰液排出；氨水引咳实验显示，与对照组比较，CBPP显著延长小鼠的咳嗽潜伏期、减少咳嗽次数（P<0.05）；qRT-PCR结果显示，与对照组比较，CBPP显著降低肺组织中Muc5AC的mRNA表达（P<0.05）。结论 CBPP具有明显的祛痰作用，促进呼吸道腺体的分泌，并促进气管纤毛运动，降低Muc5AC的含量，促进痰液排出，并且对氨水刺激引起的咳嗽有一定的镇咳作用。

Pharmacodynamic evaluation of Zhike Chuanbei Pipa Dropping Pills for expectorant and antitussive and its effect on expression of MUC5AC in lung

Objective To study the expectorant and antitussive effects of Zhike Chuanbei Pipa Dropping Pills (CBPP), and its effect on the expression of MUC5AC in lung tissue, clarify their pharmacodynamics characteristics, and provide experimental basis for the clinical positioning of this Chinese patent medicine. Methods Male SPF ICR mice were randomly divided into control group, positive drug group (ambroxol hydrochloride oral solution, 11.7 mg/kg), and CBPP low, medium and high-dose (70.2, 140.4, 280.8 mg/kg) group. Observe the expectorant effects of CBPP by phenol red expectorant experiment combined spectrophotometer method in normal mice. Rabbits were randomly divided intocontrol group, positive drug group (ambroxol hydrochloride oral solution,10.5 mg/mL) and CBPP (252 mg/mL) group. The rabbit in vitro tracheal ciliary exercise experiment was used to examine the effect of CBPP on tracheal cilia movement. The antitussive effect of CBPP of high dose by using normal mice with ammonia water induced cough experiment. Detect the content of Muc5AC mRNA in lung tissue by qRT-PCR in control and CBPP high dose group. Results Phenol red excretion experiments showed that compared with control group, the positive drug and CBPP promoted the increase of tracheal phenol red excretion in mice (P<0.05 or 0.01), and promoted the secretion of respiratory tract glands in mice, and CBPP had a certain dose-dependent effect. Rabbit in vitro tracheal ciliary exercise experiment showed treatment of positive drug and high dose of CBPP promoted cilia movement (P<0.05), and then promoted sputum excretion. Ammonia water-induced cough experiment showed that compared with control group, CBPP high-dose group prolonged the cough latency period of mice and reduced the number of coughs (P<0.05). qRT-PCR results showed that compared with control group, CBPP administration reduced the content of Muc5AC mRNA in lung tissue (P<0.05). Conclusion CBPP has obvious expectorant effect, promotes the secretion of respiratory tract glands, and promotes the movement of tracheal cilia, reduces the content of Muc5AC, promotes sputum discharge, and has a certain antitussive effect on cough caused by ammonia stimulation.