| 喉癌中p16基因突变和甲基化的研究 |
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| 引用本文： | 白素娟 高红. 喉癌中p16基因突变和甲基化的研究[J]. 中华耳鼻咽喉科杂志, 2000, 35(4): 292-294 |
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| 作者姓名： | 白素娟 高红 |
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| 作者单位： | [1]中国医科大学第2临床学院耳鼻咽喉科,沈阳 [2]基础医学院分子遗传室,沈阳 |
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| 摘 要： | 目的 探讨ｐ１６基因在喉癌发生发展中的作用和在喉癌中的失活机制。方法 应用聚合酶链反应－单链构象多态性分析（ｐｏｌｙｍｅｒａｓｅ ｃｈａｉｎ ｒｅａｃｔｉｏｎ－ｓｉｎｇｌｅ ｓｔｒａｎｄ ｃｏｎｆｏｒｍａｔｉｏｎ ｐｏｌｙｍｏｒｐｈｉｓｍａｎａｌｙ－ｓｉｓ，ＰＣＲ－ＳＳＣＰ）银染方法和ＰＣＲ甲基化敏感内匹酶方法检测３２例喉癌和相应癌旁组织的ｐ１６基因第１，第２外显子突变和甲基化热点区域部分内切酶
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| 关 键 词： | 喉肿瘤 p16基因 甲基化 基因突变 |
The study of p16 gene mutation and methylation in laryngeal squamous cell carcinoma] |
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| S Bai,H Gao,H Liang,S Fei,X Zhang,K Sun. The study of p16 gene mutation and methylation in laryngeal squamous cell carcinoma][J]. Chinese Journal of Otorhinolaryngology, 2000, 35(4): 292-294 |
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| Authors: | S Bai H Gao H Liang S Fei X Zhang K Sun |
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| Affiliation: | Department of Otorhinolaryngology, Second Clinical College, Chinese Medical University, Shenyang 110003, China. baisu@pub.sy.ln.cn |
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| Abstract: | OBJECTIVE: To investigate the development and mechanism of the p16 gene inactivation in laryngeal squamous cell carcinoma (LSCC). METHODS: With PCR-based methylation assay and PCR-SSCP, first and second exon mutation in p16 gene and methylation of endogenase in promoter in 32 cases of LSCC and paracancer tissue were studied. RESULTS: No mutation were found in first and second exon in the samples; methylation of Cfo I were detected in 6 cases in first exon, methylation of Sac II was found in 4 cases, and methylation of Hpa II in 4 cases. In second exon, methylation of Hpa II was detected in 5 cases and methylation of Cfo I in 6 cases. CONCLUSION: Methylation of promoter in p16 gene is one of the important mechanisms of the inactivation of the gene. The inactivation of p16 gene has a close relation with the development and progress of LSCC. |
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