人胎冠状动脉原位杂交c-myc和jun原癌基因表达

目的研究原癌基因c-myc和jun在人胎冠状动脉发育过程中的表达与平滑肌细胞增殖的关系.方法用原位杂交方法检测,胎龄分别为16周、22周(因治疗需要引产)的胎儿和意外死亡的足月胎儿冠状动脉前降支c-myc mRNA和jun mRNA的表达水平.杂交反应产物用图像分析仪(MIAS300)作定量分析.结果C-myc mRNA原位杂交反应产物与被测血管区域面积的百分比在16周、22周和足月胎儿分别是70、56和10;Jun mRNA的杂交信反应产物与被测血管区域面积的百分比在这三个时期分别是68、53和8.两个原癌基因在不同阶段的表达均具有显著性差异.结论本实验首次报道c-myc和jun在人胎冠状动脉发育过程中平滑肌的表达图型,c-myc和jun在胎儿冠状动脉平滑肌细胞增殖和内膜的形成过程中可能具有重要的调控作用.

Expression of proto - oncogenes c - myc and jun in human coronary artery ruring development

Objective: To investigate the expression of protooncogenes, c-myc and jun, in human coronary artery during development. Methods:In situ hybridization was employed to detect c-myc mRNA and jun mRNA in human coronary artery from aborted fetus with embryonic ages from week 16 to 22 due to treatment requirements. In addition, 3 cases of full term human fetus died of accident were also studied. Hybridized signals were quantified with a computer-assisted image-analyzing system (MIAS 300).Results: The ratio of hybridized signal of c-myc to the area of vascular wall detected were 0.7, 0.54 and 0.10 respectively corresponding to the embryonic ages, 16 weeks, 22 weeks and full term. Similar results with the ratio of 0.68, 0.53 and 0.08 for jun mRNA at above embryonic ages was also found. The levels of c-myc and jun mRNA expressed at different embryonic stage showed a significant difference.Conclusions:We first reported the expression of proto-oncogenes, c-myc and jun, in human coronary artery during embryonic development. These two proto-oncogenes may play an important role in the replication of smooth muscle cells and neonatal intima formation of human coronary artery.