| 烧伤小鼠血清对离体培养的骨髓基质细胞的影响 |
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| 引用本文： | 何佩,丁振华,郑莉,罗成基. 烧伤小鼠血清对离体培养的骨髓基质细胞的影响[J]. 实用医药杂志(山东), 2001, 18(4): 267-269 |
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| 作者姓名： | 何佩 丁振华 郑莉 罗成基 |
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| 作者单位： | 153医院核医学科!河南郑州450042(何佩)，第一军医大学防原教研室!广东广州510515(丁振华，郑莉)，第三军医大学复合伤研究所!重庆450038(罗成基) |
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| 基金项目： | 军队九五攻关指令性课题资助项目 ( 96L0 45 ) |
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| 摘 要： | 目的 研究小鼠烧伤血清对离体培养的骨髓基质细胞的影响。方法 利用流式细胞仪、HE染色等技术观察小鼠烧伤后不同时间的血清对小鼠骨髓基质细胞 (BMSC)贴壁率和基质祖细胞 (CFU F)集落形成的作用及对不同剂量的6 0 Coγ射线体外照射后的BMSC的影响。结果 小鼠烧伤后 1～ 11d的血清对基质细胞贴壁率和CFU F的形成主要表现为抑制作用 ,13～ 15d的血清主要表现为刺激作用。小鼠烧伤后 15d的血清可明显减少 5 0、70Gy照射后BMSC的凋亡和坏死。 结论 小鼠烧伤后不同时间的血清对BMSC的作用不同 ,可能与血清内细胞因子的变化有关。
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| 关 键 词： | 放射损伤 血清 骨髓基质细胞 烧伤 |
| 修稿时间： | 2001-03-09 |
Effects of the serum from burn mice on bone marrow stromal cells cultured in vitro |
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| He Pei,Ding Zhenhua,Zheng Li,et al.. Effects of the serum from burn mice on bone marrow stromal cells cultured in vitro[J]. Practical Journal of Medicine & Pharmacy, 2001, 18(4): 267-269 |
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| Authors: | He Pei Ding Zhenhua Zheng Li et al. |
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| Affiliation: | He Pei,Ding Zhenhua,Zheng Li,et al. Department of Nuclear Medicine,No.153 Hospital of PLA,Zhengzhou 450042,China |
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| Abstract: | Objective To explore the influence of the serum from burn mice on bone marrow stromal cells(BMSC)cultured in vitro.Methods Effects of the serum harvested at different time on colony formation CFU-F,stromal cell adhering-wall rate and BMSC irradiated by different dosage 60 Co γ ray were determined by flow cytometry(FCM)and HE staining.Results The serum collected from lst to 11th day showed mainly inhibition on CFU-F,stromal cell adhering-wall rate of BMSC,but showed mainly stimulation from 13th to 15th d.The serum harvested on the 15th day could reduced the apoptosis and necrosis rates of the 50 or 70Gy irradiated BMSC significantly.Conclusion The serum from burn mice at different time shows different actions on the BMSC,these actions might be correlated with change of the cytokines in the serum. |
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| Keywords: | Radiation injuries Serum Bone marrow stromal cell Burn |
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