| HPLC-DAD法测定喉疾灵胶囊中连翘苷的含量 |
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| 引用本文： | 俞永梅,张平. HPLC-DAD法测定喉疾灵胶囊中连翘苷的含量[J]. 中国药师, 2012, 15(8): 1150-1152 |
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| 作者姓名： | 俞永梅 张平 |
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| 作者单位： | 上海市松江食品药品检验所,上海,201600 |
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| 摘 要： | 目的:采用反相高效液相色谱法测定喉疾灵胶囊中连翘苷的含量。方法:采用C18色谱柱(150 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(25∶75),流速为0.8 ml.min-1,检测波长为205 nm。结果:连翘苷的线性范围为1.211～7.263μg(r=0.999 9);平均回收率为97.5%,RSD为1.4%(n=9)。结论:用本方法测定喉疾灵胶囊中连翘苷的含量,操作较简便、重现性好。
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| 关 键 词： | 连翘苷 反相高效液相色谱法 喉疾灵胶囊 含量测定 |
| 收稿时间： | 2012-03-12 |
| 修稿时间： | 2012-05-20 |
Determination of Forsythin in Houjiling Capsules by HPLC-DAD |
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| Yu Yongmei and Zhang Ping. Determination of Forsythin in Houjiling Capsules by HPLC-DAD[J]. China Pharmacist, 2012, 15(8): 1150-1152 |
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| Authors: | Yu Yongmei and Zhang Ping |
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| Affiliation: | ( Shanghai Songjiang Institute for Food and Drug Control, Shanghai 201600, China) |
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| Abstract: | Objective： To determine the content of forsythin in Houjiling capsules by HPLC-DAD. Method： The HPLC system consisted of a Cls column（ 150 mm × 4.6 mm ,5 μm）and acetonitrile-0.1% phosphate solution （25：75 ） as the mobile phase with UV detection wavelength at 205 nm and flow rate of 0.8 ml min-1. Result： The linear range was 1.211-7. 263 μg（ r = 0.999 9 ） , and the average recovery was 97.5% （RSD = 1.4%, n =9）. Conclusion： The method is simple, specific and accurate. |
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| Keywords: | Forsythin RP - HPLC Houjiling capsules Content determination |
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