rhIGF-1对成骨细胞增殖、分化及骨保护素、骨保护素配体基因mRNA表达的影响

目的 观察不同剂量rhIGF-1对成骨细胞增殖，分化及骨保护素，骨保护素配体mRNA基因表达的影响，为明确骨保护素，骨保护素配体在骨质疏松症发病的作用机理及rhIGF-1在临床中应用提供实验依据。方法 取2代培养的大鼠成骨细胞，在rhIGF-1为0ng/ml,10ng/ml,20ng/ml,50ng/ml的浓度中培养。观察细胞的生长及钙结节的形成，MTT法，碱性磷酸酶（ALP），骨钙素（OCN）测定细胞增殖和分化，RT－PCR测定rhIGF-1对成骨细胞骨保护素，骨保护素配体基因mRNA表达的影响。结果 成骨细胞在7d可铺满瓶壁，30d可形成钙结节，rhIGF-1可促进成骨细胞的增殖，ALP和OGN的分泌，促进骨保护素，骨保护素配体基因的表达，以促进骨保护素表达明显，在rhIGF-1为10ng/ml时作用明显。结论 rhIGF-1可促进大鼠成骨细胞的增殖，分化，及骨保护素，骨保护素配体基因mRNA的表达，骨保护素mRNA表达显。rhIGF-1可能通过影响骨保护素，骨保护素配体而调节成骨细胞破骨细胞的平衡，使骨重建，从而防治骨质疏松症。

Effect of rhIGF-1 on the proliferation and differentiation and OPG,RANKL gene expression in rat osteoblast

Objective To assess the effect of rhIGF 1 on the proliferation and differentiation and OPG,RANKL gene expression in rat osteoblast,investigate the mechanism of OPG,RANKL in osteoporosis Methods The second generation of rats osteoblast were cultured in medium with different concentrations of rhIGF 1 Cell proliferation was detected by optical microscope and MTT method, differentiation was determined by measuring alkaline phosphatase (ALP) activity and osteocalcin secretion OPG and RANKL gene expression were quantified by RT PCR Results rhIGF 1 in low dose ( P <0 05) obviously increased osteoblast proliferation and differentiation and the gene espression of OPG and RANKL, especially in the gene expression of OPG (P <0 05) Conclusion rhIGF 1 stimulated osteoblast proliferation and defferentiation especially in low dose, so as in the gene espression of OPG and RANKL It increased more the gene expression of OPG than that of RANKL rhIGF 1 regulated the balance between osteoblast and osteoclast by affecting the gene expression of OPG and RANKL, it is possibly the protecting and treatment mechanism of osteoporosis