碳酸铵对柑橘酸腐病菌的抑制效果及作用机制

由酸腐病菌引起的酸腐病是柑橘重要的采后病害,目前缺少有效控制酸腐病的杀菌剂.本研究分析了碳酸铵作为通常认为安全的药剂对酸腐病菌的抑菌活性和可能的作用机制.结果显示:碳酸铵可以抑制孢子萌发和芽管伸长,抑制菌丝生长,并且抑制效应与质量浓度呈正相关,在0.8 g/L条件下完全抑制孢子萌发和菌丝生长.进一步分析表明,碳酸铵抑制...

Inhibitory Effect and Action Mechanism of Ammonium Carbonate against Sour Rot in Citrus Fruit

Sour rot,caused by Geotrichum citri-aurantii,is one of the most devastating diseases of harvested citrus fruit.Nonetheless,there are currently no efficient fungicides available to control this disease.In this study,ammonium carbonate as a generally recognized as safe compound was evaluated for its antifungal activity against the pathogen and the possible mechanism of its action was investigated.The results showed that ammonium carbonate could inhibit spore germination,germ tube elongation and mycelial growth of G.citri-aurantii in vitro in a dose-dependent manner,completely inhibiting spore germination and mycelial growth at a concentration of 0.8 g/L.Further analysis revealed that ammonium carbonate inhibited spore activity,powerfully repressed mycelial respiration,altered the permeability of cell membrane as indicated by propidium iodide(PI)staining,and gradually increased the leakage of intracellular ions,proteins and nucleic acids,thus leading to the functional loss of hyphal cells.In vivo experiment demonstrated that 20 g/L ammonium carbonate could effectively decrease lesion diameter in Valencia oranges artificially inoculated with a spore suspension at 1×10^6 spores/mL,20μL and reduce disease incidence.These results suggested that ammonia carbonate could be a potential alternative to fungicides to control sour rot in citrus fruit.