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| 7-羟基黄酮及磷酰化7-羟基黄酮与DNA的弱相互作用荧光法研究 | |
| 引用本文： | 郭玉华,陈晓岚,张婷,屈凌波,赵玉芬,郁有祝.7-羟基黄酮及磷酰化7-羟基黄酮与DNA的弱相互作用荧光法研究[J].光谱学与光谱分析,2006,26(3):475-479. |
| 作者姓名： | 郭玉华 陈晓岚 张婷 屈凌波 赵玉芬 郁有祝 |
| 作者单位： | 1. 郑州大学化学系, 河南省化学生物与有机化学重点实验室, 河南 郑州 450052 2. 安阳师范学院，河南 安阳 455002 3. 清华大学教育部生命有机磷化学和化学生物重点实验室，北京 100084 |
| 摘 要： | 以溴化乙锭(EB)为荧光探针，研究了7-羟基黄酮及磷酰化7-羟基黄酮与DNA的弱相互作用。实验结果表明，两种化合物与DNA间均存在弱相互作用，但与7-羟基黄酮相比，磷酰化7-羟基黄酮对DNA更具亲和力。随着温度的升高，7-羟基黄酮及磷酰化7-羟基黄酮对DNA-EB体系的荧光猝灭常数降低，两种化合物均可与DNA形成复合物，此猝灭过程为静态猝灭。根据Stern-Volmer方程和Scatchard方程，常温下7-羟基黄酮及磷酰化7-羟基黄酮对DNA-EB体系的荧光猝灭常数和它们与DNA的固有的结合常数分别为：Kq1=601 L·mol-1,Kq2=1 381 L·mol-1;K1=2.07×104 L ·mol-1,K2=3.19×104 L·mol-1。 |
| 关 键 词： | EB探针 DNA 7-羟基黄酮 磷酰化7-羟基黄酮 荧光猝灭 弱相互作用 |
| 文章编号： | 1000-0593（2006）03-0475-05 |
| 收稿时间： | 2004-12-26 |
| 修稿时间： | 2005-05-11 |
Study on the Non-Covalent Interaction of 7-Hydroxy Flavone and Its Phosphate with DNA by Fluorescence Method |
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| GUO Yu-hua,CHEN Xiao-lan,ZHANG Ting,QU Ling-bo,ZHAO Yu-fen,YU You-zhu.Study on the Non-Covalent Interaction of 7-Hydroxy Flavone and Its Phosphate with DNA by Fluorescence Method[J].Spectroscopy and Spectral Analysis,2006,26(3):475-479. | |
| Authors: | GUO Yu-hua CHEN Xiao-lan ZHANG Ting QU Ling-bo ZHAO Yu-fen YU You-zhu |
| Affiliation: | 1. Key Laboratory for Bio-Chemistry and Organic Chemistry, Zhengzhou University, Zhengzhou 450052, China2. Anyang Teachers College, Anyang 455002, China3. Key Laboratory for Bio-organic Phosphorus Chemistry, Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, China |
| Abstract: | The non-covalent interaction of 7-hydroxy flavone and its phosphate with DNA was studied using ethidium bromide (EB)as a probe. The result showed that both 7-hydroxy flavone and its phosphate could form non-covalent complexes, but the phosphorylated flavonoid showed higher binding affinity with DNA than 7-hydroflavone did. Experiments demonstrated that the higher the temperature, the lower the slop of quenching curve of DNA-EB in the presence of different amounts of 7-hydroxy flavone and its phosphate. It was confirmed that the combinations of DNA with 7-hydroxy flavone and its phosphate were a single static quenching process. According to the Stern-Volmer equation and Scatchard equation the quenching constants and the intrinsic binding constants of 7-hydroxy flavone and its phosphate were measured respectively, they were Kq1 = 601 L x mol(-1), Kq2 = 1381 L x mol(-1); K1 = 2.07 x 10(4) L x mol(-1) , K2 = 3.19 x 10(4) L x mol(-1) respectively. |
| Keywords: | EB probe DNA 7-hydroxy flavone Diethyl flavon-7-yl phosphate Fluorescence quenching Non-covalent interaction |
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