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| 高致病性猪繁殖与呼吸系统综合症病毒荧光定量PCR检测方法的建立 | |
| 引用本文： | 刘园园,肖性龙,吴晖,余以刚,张经纬,程邦照,龚俊,杨晓泉,刘冬梅. 高致病性猪繁殖与呼吸系统综合症病毒荧光定量PCR检测方法的建立[J]. 现代食品科技, 2008, 24(7): 731-734 |
| 作者姓名： | 刘园园 肖性龙 吴晖 余以刚 张经纬 程邦照 龚俊 杨晓泉 刘冬梅 |
| 作者单位： | 1. 华南理工大学轻工与食品学院,广东,广州,510640 2. 华南理工大学轻工与食品学院,广东,广州,510640深圳太太基因工程有限公司,广东,深圳,51805 3. 深圳太太基因工程有限公司,广东,深圳,51805 |
| 摘 要： | 为了对当前爆发流行的高致病性猪繁殖与呼吸系统综合症病毒建立快速准确的检测方法,根据该类病毒在Nsp2基因1594-1680处缺失87个碱基的特点,设计了一对特异性引物和一个Taqman探针,通过对反应条件的优化,建立了荧光定量PCR检测方法.该方法特异性强,灵敏度高,能很好地区分高致病性猪繁殖与呼吸系统综合症病毒和其它病毒,没有发现假阳性和假阴性现象,检测病毒滴度达到1TCID50.用该法对38份疑似样本进行检测,阳性率60.5%,与常规PCR法符合率100%. |
| 关 键 词： | 高致病性猪繁殖与呼吸系统综合症 荧光定量PCR 检测 |
| 收稿时间： | 2008-03-24 |
Development of Fluorescence Quantitative PCR Assay for the virulent Porcine Reproductive and Respiratory syndrome virus |
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| LIU Yuan-yuan,XIAO Xing-long,WU Hui,YU Yi-gang,ZHANG Jing-wei,CHENG Bang-zhao,GONG Jun,YANG Xiao-quan,LIU Dong-mei. Development of Fluorescence Quantitative PCR Assay for the virulent Porcine Reproductive and Respiratory syndrome virus[J]. Modern Food Science & Technology, 2008, 24(7): 731-734 | |
| Authors: | LIU Yuan-yuan XIAO Xing-long WU Hui YU Yi-gang ZHANG Jing-wei CHENG Bang-zhao GONG Jun YANG Xiao-quan LIU Dong-mei |
| Affiliation: | (1.College of Light Industry & Food Science, South China University of Technology, Guangzhou 510640, China);(1.College of Light Industry & Food Science, South China University of Technology, Guangzhou 510640, China)(2.Shenzhen Taitai Genomics, Inc., Shenzhen 518057, China) |
| Abstract: | A method for rapid detection of the highly virulent Porcine Reproductive and Respiratory syndrome virus (H-PRRSV) was developed using fluorescence quantitative PCR assay (FQ-PCR). The Taqman probe and a pair of specific primers and were designed based on Nsp2 gene with 87-base-pairs sequence deletion. Then the FQ-PCR method was developed by optimization of the reaction conditions, which showed high specificity and sensitivity, and well differentiated H-PRRSV form other viruses. 1TCID50/ml of the H-PRRSV could be detected by this method. Besides, similar positive percentages of 38 tissue specimens (60.5%) were found using FQ-PCR assay or conventional PCR assay. |
| Keywords: | highly virulent Porcine Reproductive and Respiratory syndrome virus (H-PRRSV) fluorescence quantitative PCR (FQ-PCR) detection |
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