促甲状腺素释放激素对失血致低血容量性休克大鼠心肺功能的影响

目的：探讨促甲状腺素释放激素（ＴＲＨ）抗失血致低血容量性休克的作用和对呼吸频率（ＲＲ）、平均动脉压（ＭＡＰ）、血气参数及酸碱平衡紊乱的影响。方法：复制失血致低血容量性休克大鼠模型，观察ＴＲＨ治疗前、后及各组各时间点大鼠ＲＲ、ＭＡＰ、血气和酸碱平衡等各参数的变化。结果：ＴＲＨ组给药后ＭＡＰ即刻升高，５分钟ＭＡＰ达（１４．０±１．３）ｋＰａ（１ｋＰａ＝７．５ｍｍＨｇ），较低血容量性休克（ＨＳ）组增高３２％（ｔ＝２．５３１，Ｐ＜０．０５）；休克组大鼠在伤后３６０分钟ＭＡＰ仍较低，ＴＲＨ治疗组ＭＡＰ还比休克组高４２％（ｔ＝３．０１４，Ｐ＜０．０５）。休克组和ＴＲＨ治疗组失血后ＲＲ明显减慢，ＴＲＨ给药后５分钟ＲＲ升为（８０±９）次／ｍｉｎ，较休克组升高７８％（ｔ＝３．８９２，Ｐ＜０．０１）；伤后３６０分钟，ＴＲＨ组ＲＲ达（９９±９）次／ｍｉｎ，比休克组高７１％（ｔ＝３．４５６，Ｐ＜０．０１）。ＴＲＨ降低了肺体指数和肺含水率；升高ｐＨ、ＨＣＯ－３，改善酸中毒和酸碱失衡。结论：ＴＲＨ在失血致低血容量性休克治疗过程中有重要作用，能有效减轻休克的严重程度。

Effects of thyrotropin releasing hormone on cardiac and pulmonary function in rats following hemorrhagic shock

Objective :To study the effects of thyrotropin releasing hormone (TRH) on changes in respiratory rhythm,mean arterial pressure (MAP),blood gases,and the acid base equilibrium after hemorrhagic shock. Methods :Hypovolemic shock was induced in Wistar rats by carotid artery bleeding to maintain MAP at 60 kPa (1 kPa=75 mmHg)for an hour.21 rats were randomly divided into three groups.group 1 (NS,n=7) served as normal controls,group 2 (HS,n=7) as hemorrhagic shock,and group 3(TRH,n=7) as TRH HS treatment.Blood gases,MAP,respiratory rhythm,lung/body weight index (LBI),and rate of lung water (RLW) were determined. Results :At 5 minutes after treatment with TRH,MAP increased to (14013)kPa,which was elevated by 32% compared to HS group (P<005).MAP maintained a low value in HS group 360 minutes after hemorrhage,and it was much lower than that in TRH treated group (P<005).In both HS and TRH groups,respiratory rhythm reduced following hemorrhage,while it increased by 78% and 71% in animals treated with TRH at and 360 minutes respectively (808)beats/min and(999)beats/min,both P<001.Moreover,it was found that TRH markedly increased blood pH and HCO-3 values,reduced PaCO2 and LBI and RLW,and improved acid base imbalance in rats following shock. Conclusions :TRH plays an important role in the treatment of hypovolemic shock,as it can effectively attenuate the severity of shock.