北疆部分地区食源性大肠杆菌血清型、毒力基因及耐药基因的研究

目的研究北疆部分地区食源性大肠杆菌的优势血清型、毒力基因和耐药基因的相关性。方法采用玻片凝集法测定了大肠杆菌血清型分布,PCR方法调查11种耐药基因和9种毒力基因。结果血清试验,定型菌株26株,分别属于17种血清型,其中O1、O115为优势血清型;PCR结果表明,不含有所检测耐药基因的分离株占12.50%,至少含有2种以及以上的分离株占50.00%,分离株对blaOXA基因携带率高达57.14%;检出率较高的毒力基因为fimC(64.29%)和fimA(25.00%)。结论 O1、O115为主要的血清型,2种血清型菌株拥有的耐药基因谱和毒力基因谱不相同。

Study on serotypes, virulence genes and resistance genes of Escherichia coli isolated from food in parts of Northern Xinjiang

Objective The aim of the study was to investigate the prevalence of serotypes, virulence genes and resistance genes of Escherichia coli isolated from food in parts of Northern Xinjiang. Methods O serogroups were identified by agglutination with specific antisera, all strains were screened for 11 resistance genes and 9 virulence genes by PCR. Results Serum tests showed , seventeen O serogroups were identified for 26 isolates, of which O1 and O115 were dominant serogroups; PCR results showed that isolates of not containing the detected resistance genes accounted for 12.50% , isolates of containing at least two and more accounted for 50.00%, carrying rate of blaOXA gene reached up 57.14%; fimC gene(64.29%)and fimA(25.00%)gene accounted for higher detection rate in virulence genes .Conclusion It is concluded that O1 and O115 are dominant serogroups ,two serogroups had different resistance genes and virulence genes.