艾灸足三里等穴诱导HSP60对急性胃黏膜损伤大鼠Smac表达的影响

目的观察艾灸足阳明胃经"足三里"等穴对急性胃黏膜损伤模型大鼠HSP60、Smac表达的影响,探讨艾灸足阳明经穴抗胃黏膜损伤的保护性机制。方法 24只SD大鼠随机分为空白对照组、模型对照组、艾灸穴位组,8只/组。大鼠艾灸预处理8 d,进行无水乙醇灌胃,制作急性胃黏膜损伤大鼠动物模型。用免疫组化方法检测HSP60及Smac表达。结果与空白对照组相比,模型对照组大鼠Smac、HSP60表达均显著升高差异有统计学意义(P<0.01);艾灸穴位组HSP60表达明显上调(P<0.01),而Smac表达明显被抑制(P<0.01)。与模型对照组相比,艾灸穴位组Smac表达明显下降(P<0.01),HSP60表达显著提高差异有统计学意义(P<0.01)。结论艾灸足阳明经穴预处理可以诱导保护性蛋白HSP60的过度表达,抑制促凋亡因子Smac表达,从而起到保护胃黏膜损伤的作用。

Effects of moxibustion on acupoint Zusanli and the expressions of HSP60 and Smac in acute gastric mucosal lesion of rats

Objective To observe the effects of moxibustion pre-treatment at Zusanli and Zhongwan acupoints on expressions of heat shock protein(HSP)60 and Smac in a rat model of acute gastric mucosal lesion(AGML) and explore the underlying protective mechanisms of moxibustion for gastric mucosal lesion.Methods A total of 24 Sprague Dawley rats were divided into 3 groups,i.e.blank,model and moxibustion acupoint(n=8 each).After an 8-day moxibustion pretreatment,the gastric lavage of anhydrous ethanol was used to establish the AGML model.The immunohistochemistry was used to examine the expressions of HSP60 and Smac.Results As compared with the blank group,the expressions of Smac and HSP60 were markedly elevated in the model groups(P<0.01);The expressions of HSP60 was markedly elevated in the moxibustion acupoint group(P<0.01) while those of Smac became obviously suppressed(P<0.01).As compared with the model group,the expressions of Smac decreased significantly in the moxibustion acupoint group(P<0.01) while those of HSP60 increased markedly(P<0.01).Conclusion The pre-treatment of moxibustion could alleviate the gastric mucosal lesion caused by anhydrous ethanol,induce the over-expressions of HSP60 and down-regulate the expression of Smac so as to protect against gastric mucosal lesion.