MALDI ion trap mass spectrometer with charge detector for large biomolecule detection |
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Authors: | Chen Chien-Hsun Lin Jung-Lee Chu Ming-Lee Chen Chung-Hsuan |
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Affiliation: | Genomics Research Center, Academia Sinica, Taipei, Taiwan. |
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Abstract: | Up to now, all commercial matrix-assisted laser desorption/ionization (MALDI) mass spectrometers still can not efficiently analyze very large biomolecules. In this work, we report the development of a novel MALDI ion trap mass spectrometer which can enrich biomolecular ions to enhance the detection sensitivity. A charge detector was installed to measure the large ions directly. With this design, we report the first measurement of IgM with the mass-to-charge ratio (m/z) at 980?000. In addition, quantitative measurements of the number of ions can be obtained. A step function frequency scan was first developed to get a clear signal in the m/z range from 200,000 to 1,000,000. |
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