Determination of norfloxacin in pharmaceuticals,human serum,and urine using a luminol—dissolved oxygen chemiluminescence system |
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Authors: | X D Shao H Y Liu X F Gao W Q Chen Z H Song |
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Affiliation: | (1) Department of Chemistry, Northwest University, Xi’an, 710069, China |
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Abstract: | A sensitive chemiluminescence method, based on the enhancive effect of norfloxacin on the reaction between luminol and dissolved
oxygen in a flow injection system, was proposed for the determination of norfloxacin. The increment of the chemiluminiscence
intensity was proportional to the concentration of norfloxacin, giving a calibration graph linear over the concentration from
0.4 ng mL−1 to 400.0 ng mL−1 (r
2 = 0.9988) with the detection limit of 0.1 ng mL−1 (3 × σ
noise). At the flow rate of 2.0 mL min−1, a complete determination of norfloxacin, including sampling and washing, could be accomplished in 30 s with the relative
standard deviation lower than 3.0 %. The proposed method was applied successfully to determine norfloxacin in pharmaceuticals,
human urine, and serum. Possible mechanism of the reaction was also discussed. |
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Keywords: | norfloxacin chemiluminescence luminol serum urine flow-injection |
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