胰腺癌中DPC4/Smad4、nm23-H1/NDPK的表达及其意义

目的探讨nm23-H1/NDPK、DPC4/Smad4蛋白在人胰腺癌组织中的表达情况及其相互关系.方法采用免疫组织化学SABC方法.实验组为34例胰腺癌组织,对照组为慢性胰腺炎组织34例.结果 DPC4/Smad4蛋白在实验组中的阳性表达低于对照组织,差异有显著性意义(P＜0.05).nm23-H1/NDPK蛋白在实验组中的阳性表达明显高于在对照组织中的表达,两者差异有显著性(P＜0.05).而且在高分化组织、无淋巴结转移组、临床分期为I、II期组织中的阳性表达低于低分化组织、有淋巴结转移及临床分期为Ⅲ、Ⅳ期组织中的表达,其差异有统计学意义(P＜0.05).结论⑴nm23-H1/NDPK基因可能是胰腺癌恶性程度的指标,也可能是转移与预后差的指标,nm23-H1/NDPK在胰腺癌中可能不显示肿瘤转移抑制功能.⑵DPC4/Smad4作为一种抑癌基因,其改变对胰腺癌发生发展可能起重要作用.⑶DPC4/Smad4与nm23-H1/NDPK在胰腺癌中表达不相关.

The Expression and Their Clinical Significance of DPC4/ Smad4 and nm23-H1/NDPK in Pancreatic Carcinoma

Objective To explore the expression and significance of DPC 4/Smad 4 and nm23-H1/NDPK in human pancreatic carcinoma. Methods The expressions of DPC 4/Smad 4 and nm23/NDPK were detected by immunohistochemical method in 34 pancreatic carcinoma tissues and 34 chronic pancreatitis tissues. Results The positive rate of DPC 4/Smad 4 expression was significantly higher in the chronic pancreatitis than in the pancreatic carcinoma(P<0.05). The positive rate of nm23-H1/NDPK expression was significantly lower in the pancreatic carcinoma than in the chronic pancreatitis(P<0.05). nm23-H1/NDPK expression in the pancreatic carcinoma was obviously correlated with the lymph node metastasis and pathologic differential degree of tumor. The positive rate of nm23-H 1/NDPK expression in the pancreatic carcinomas of higher differentiation and without lymph node metastasis was lower than that in the pancreatic carcinomas of lower differentiation and with lymph node metastasis (P<0.05). Conclusion The expressions of DPC 4/Smad 4 and nm23- H1/ NDPK may be related with the pathogenesis of pancreatic carcinomas. nm23-H1/NDPK may not have the function of inhibiting pancreatic cancer metastasis. There was not relationship between DPC 4/Smad 4 and nm23-H1/NDPK expressions in pancreatic carcinoma.