| 提高重组TRAIL表达产率的优化策略 |
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| 引用本文： | 鲍熹珺,魏东芝,沈亚领,周劲松,张国钧,孙菁,球谊.提高重组TRAIL表达产率的优化策略[J].中国生物工程杂志,2009,29(10):74-80. |
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| 作者姓名： | 鲍熹珺 魏东芝 沈亚领 周劲松 张国钧 孙菁 球谊 |
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| 作者单位： | 1.华东理工大学生物反应器工程国家重点实验室 鲁华生物技术研究所 上海 200237
2.上海恰尔生物技术有限公司 上海 201103 |
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| 基金项目： | 上海市重点学科建设项目 |
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| 摘 要： | 肿瘤坏死因子相关凋亡诱导配体（Apo2L/TRAIL）是一种新型的抗肿瘤蛋白。针对缩短发酵周期，提高生产效率这一目标，首先在摇瓶中利用响应面设计优化发酵表达条件，总TRAIL蛋白的表达量提高到25.7%。在此基础上研究了发酵条件对可溶性TRAIL蛋白表达量的影响。于3.7L发酵罐放大进行补料-分批发酵实验时，单位菌体可溶性TRAIL蛋白的表达量提高了67%，实现了在大肠杆菌高密度培养过程中单位细胞重组TRAIL蛋白的可溶性表达和体积生产率的提高。
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| 关 键 词： | 肿瘤坏死因子相关凋亡诱导配体 重组大肠杆菌 响应面法 发酵优化 |
| 收稿时间： | 2009-04-23 |
| 修稿时间： | 2009-07-30 |
Optimization of Fermentation Conditions for High Expression Productivity of Recombinant TRAIL |
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| BAO Xi-Jun,WEI Dong-zhi,SHEN Ya-ling,ZHOU Jin-song,ZHANG Guo-jun,SUN Jing,QIU Yi.Optimization of Fermentation Conditions for High Expression Productivity of Recombinant TRAIL[J].China Biotechnology,2009,29(10):74-80. |
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| Authors: | BAO Xi-Jun WEI Dong-zhi SHEN Ya-ling ZHOU Jin-song ZHANG Guo-jun SUN Jing QIU Yi |
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| Abstract: | Tumor necrosis factor related apoptosis inducing ligand(TRAIL) is a novel anticancer therapeutic agent. In order to reduce the incubation time and enhance the production efficiency, firstly the response surface method was used to optimize the fermentation and expression condition of TRAIL in shake flasks. As a result, the expression of the total TRAIL protein reached 25.7%. Based on this, the influence of fermentation condition to the soluble TRAIL protein expression was studied. The recombinant strains were cultured in 3.7L fermenter and the expression of soluble TRAIL protein per gram cell enhanced 67%. That realized the high soluble expression of TRAIL protein per gram cell in high cell density and the enhancement of volume productivity. |
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| Keywords: | Apo2L/TRAIL Recombinant Escherichia coli Response surface Fermentation optimization |
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