Kinetik der hepatischen Farbstoffaufnahme von Indocyaningrün. Einfluss von Bilirubin und Natriumglykocholat |
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Authors: | Dr G Paumgartner Dr J Huber G Grabner |
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Affiliation: | (1) II. Medizinische Universitätsklinik Wien, Garnisongasse 13, 1090 Wien, österreich;(2) Kantonsspital, 8200 Schaffhausen, Schweiz |
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Abstract: | Summary Kinetics of hepatic uptake of indocyanine green, a dye which is used for evaluation of liver function, were studied in the rat. The results indicate that the relationship between ICG-dose and initial hepatic dye uptake obeys Michaelis-Menten kinetics suggesting an interaction of the dye with a carrier or fixed site in the liver cell. Thus it was possible to calculate maximum ICG-uptake (v
max
) and the Michaelis constant (K
m
) of this transport system from several submaximal values.v
max
was 7.65 (6-06-9.65)22 mg per 100 g liver/min and K
m
0.56 (0.31–0.81)22. Under the influence of substances which inhibit the elimination of dyes by the liver the parametersv
max
and K
m
showed changes which allowed characterization of the type of inhibition. While sodium glycocholate had no influence on maximum hepatic ICG-uptake and the Michaelis constant bilirubin caused a significant increase of K
m
to 1.29 (0.68–1.90)22 without significantly changingv
max
. These data suggest that bilirubin interferes with hepatic uptake of indocyanine green by competitive inhibition and that uptake of bile acids is dependent on a different mechanism. |
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Keywords: | |
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