黑花生衣色素的提取及调节C2C12肌管细胞葡萄糖消耗的作用研究

从花生加工中的副产物黑花生衣中提取纯化黑花生衣色素(BPSP),并初步探究其相关细胞生物学活性.首先,采用紫外可见(UV-vis)全光谱扫描及分光光度法对BPSP的检测条件、对温度和pH稳定性以及最佳提取条件进行了探究,发现BPSP在pH2～3、520nm可见波长时有最佳特征吸收峰,其在水溶液中比在乙醇溶液中更为稳定,...

Extraction of black peanut skin pigment and its regulation of glucose consumption in C2C12 myotubes

Black peanut skin pigment(PBSP)was extracted and purified from the black peanut skins which were regarded as a by-product of the peanut industry,and its related cell biological activities were preliminarily studied.First,the detection conditions,temperature and pH stability and optimum extraction conditions of BPSP were explored by UV-vis full-spectrum scanning technique and spectrophotometry.Results showed that BPSP has optimal characteristic absorption peak at pH2~3 and 520nm visible wavelength,and it is more stable in aqueous solution than in ethanol solution;also,BPSP aqueous showed the best stability when the temperature is not higher than 50℃at pH2.0;in addition,the optimal extraction conditions of BPSP were as follows:70℃,pH2.0 acidic aqueous solution extract for 15min;under this condition,the yield of BPSP was 7.95mg/g dry black peanut coat weigh.Further,BPSP anthocyanins were purified by ethyl acetate(EtOAc)partition combined with macroporous resin column chromatography technique,and the purity of total anthocyanins was increased nearly by 5-folds,from 5.2%(raw extraction)to 25.4%(after purification).Finally,purified BPSP was used to intervene the differentiated mature C2C12 myotubes.It was found that BPSP at the dose of 5μg/mL could significantly increase the glucose consumption of C2C12 myotube cells(P<0.05),moreover,within the concentration range of 1~5μg/mL,this promoting effect was dose-dependent.