茶酒糟中茶多酚提取工艺优化及其抗氧化活性的研究

该试验以茶酒糟为原料，乙醇为提取溶剂，采用水浴振荡法提取茶多酚，通过单因素试验探究乙醇体积分数、料液比、提取时间、温度对茶多酚提取量的影响，在此基础上，通过正交试验优化茶多酚的水浴振荡乙醇提取工艺，并考察茶多酚对羟自由基（·OH）、1,1-二苯基-2-三硝基苯肼自由基（DPPH·）的清除能力。结果表明，从茶酒糟中提取茶多酚的最佳提取工艺是：乙醇体积分数60%，料液比1∶30（g∶mL），提取时间12 min，提取温度75 ℃。在此优化条件下，茶多酚的提取量为41.52 mg/g。从茶酒糟提取得到的茶多酚对·OH和DPPH·最大清除率分别达到91.7%、93.7%，表明茶多酚具有一定的清除自由基的能力，具有较强的抗氧化活性。

Optimization of extraction process and antioxidant activity of tea polyphenols from tea distiller's grains

Using tea distiller's grains as raw material and ethanol as extraction solvent,tea polyphenols were extracted by water bath oscillation method.The effects of ethanol volume fraction,solid-liquid ratio,extraction time and temperature on the extraction amount of tea polyphenols were investigated by single factor experiments.On the basis of single factor test,the extraction process of tea polyphenols using water bath ethanol was optimized by orthogonal tests,and the scavenging ability of tea polyphenols to hydroxyl radical(·OH)and 1,1-diphenyl-2-trinitrophenyl radical(DPPH·)was determined.The results showed that the optimal extraction process for tea polyphenols from tea distiller's grains were as follows:ethanol volume fraction 60%,solid-liquid ratio 1∶30(g∶ml),extraction time 12 min,and temperature 75℃.Under these optimal conditions,the extraction amount of tea polyphenols was 41.52 mg/g.The maximum scavenging rates of·OH and DPPH·were 91.7%and 93.7%,respectively,indicating that tea polyphenols had a certain ability of scavenging free radical and had strong antioxidant activity.