| 迟钝爱德华氏菌FimA基因的克隆及序列分析 |
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| 引用本文： | 郭立新,李寿崧,江树勋,陈文炳,邵碧英,杨婕.迟钝爱德华氏菌FimA基因的克隆及序列分析[J].食品科学,2006,27(11):45-48. |
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| 作者姓名： | 郭立新 李寿崧 江树勋 陈文炳 邵碧英 杨婕 |
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| 作者单位： | 福建出入境检验检疫局 |
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| 基金项目： | 福建省科技厅科技攻关计划重点项目（2004Y046）;国家质检总局科技司资助项目（2006IK115） |
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| 摘 要： | 以迟钝爱德华氏菌Et、XA、EA分离株的DNA为模板,采用PCR技术,扩增菌毛亚基(FimA)基因的DNA片段,将其克隆到pMD18-T载体上。通过序列测定,分析结果表明:Et、XA、EAFimA基因由534个核苷酸组成,编码177个氨基酸。Et、XA、EA之间FimA基因核苷酸同源性为99%,与其它分离物核苷酸同源性为76%～77%,氨基酸同源性为81%～82%。
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| 关 键 词： | 迟钝爱德华氏菌 菌毛亚基 克隆 序列分析 |
| 文章编号： | 1002-6630（2006）11-0045-04 |
| 收稿时间： | 2006-06-25 |
| 修稿时间： | 2006-06-25 |
Cloning and Sequence Analysis to Fimbrial Subunit Gene (fimA) of Edwardsiella tarda |
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| GUO Li-xin,LI Shou-song,JIANG Shu-xun,CHEN Wen-bing,SHAO Bi-ying,YANG Jie.Cloning and Sequence Analysis to Fimbrial Subunit Gene (fimA) of Edwardsiella tarda[J].Food Science,2006,27(11):45-48. |
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| Authors: | GUO Li-xin LI Shou-song JIANG Shu-xun CHEN Wen-bing SHAO Bi-ying YANG Jie |
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| Affiliation: | Fujian Entry-exit Inspection and Quarantine Bureau of PRC, Fuzhou 350001, China |
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| Abstract: | Fimbrial subunit gene (fimA) of Et, XA and EA isolates were cloned and sequenced. The sequence analysis showed that the fimbrial subunit gene of Et, XA and EA isolates contained 534 nucleotides, encoding a protein of 117 amino acids. The similarity of nucleotide (nt) sequence of fimA gene among Et, XA and EA isolates were 99%. The nucleotide and amino acid(aa) sequence of fimA gene of these isolates shared 76% to 77% and 81% to 82% similarities to that of other Edwardsiella tarda isolates, respectively. |
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| Keywords: | Edwardsiella tarda FimA cloning sequence analysis |
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