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| 同型半胱氨酸对人脐静脉内皮细胞eNOS表达及NO含量的影响 | |
| 引用本文： | 徐倩,周晓慧,曹凯,牛成伟,王冰.同型半胱氨酸对人脐静脉内皮细胞eNOS表达及NO含量的影响[J].中国老年学杂志,2011,31(21). |
| 作者姓名： | 徐倩 周晓慧 曹凯 牛成伟 王冰 |
| 作者单位： | 1. 承德医学院,河北承德,067000 2. 上海中医药大学 3. 承德医学院附属医院 |
| 基金项目： | 河北省教育厅科学研究计划项目(No.2003124) |
| 摘 要： | 目的 通过观察同型半胱氨酸(Hcy)对人脐静脉内皮细胞(HUVECs)一氧化氮合酶(eNOS)表达及NO含量的影响,探讨Hcy诱导动脉粥样硬化形成的病理机制.方法 将体外培养的人脐静脉内皮细胞分为5组,分别加入Hcy0、2.5、5、10及15 mmol/L,培养24h.通过MTT比色法测定细胞活力,采用RT-PCR法检测eNOS mRNA的表达,通过硝酸还原酶法测NO含量.结果 与对照组比较,Hcy(5 ～ 15 mmol/L)剂量处理组细胞活力明显下降(P＜0.05,P＜0.01),eNOS mRNA的表达显著降低(P＜0.01)；NO的含量明显下降(P＜0.01),eNOS mRNA的表达变化及NO的含量变化呈现明显的剂量依赖性(P＜0.01).结论 Hcy可通过抑制eNOS mRNA的表达,使eNOS合成减少,活性降低,进而减少HUVECs内NO的生成,这可能是Hcy诱导动脉粥样硬化形成的病理机制之一. |
| 关 键 词： | 人脐静脉内皮细胞 同型半胱氨酸 四甲基噻唑氮蓝 NO 内皮型一氧化氮合酶 |
Effects of homocysteine on the expression of eNOS and the production of NO in the human umbilical veins endothelial cells |
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| XU Qian,ZHOU Xiao-Hui,CAO Kai,et al..Effects of homocysteine on the expression of eNOS and the production of NO in the human umbilical veins endothelial cells[J].Chinese Journal of Gerontology,2011,31(21). | |
| Authors: | XU Qian ZHOU Xiao-Hui CAO Kai |
| Affiliation: | XU Qian,ZHOU Xiao-Hui,CAO Kai,et al.Chengde Medical College,Chengde 067000,Hebei,China |
| Abstract: | Objective To investigate the effects of homocysteine on the expressions of eNOS and the production of NO in the human umbilical veins endothelial cells(HUVECs) and explore the role of homocysteine in the development of atherosclerosis.Methods The HUVECs were randomly divided into 5 groups,and each group was cultured with 0,2.5,5,10,15 mmol/L Hcy for 24 hours.MTT assay was used to detect cell viability.The expression of eNOS mRNA was tested by RT-PCR.The method of nitric acid reductase was used to measure th... |
| Keywords: | Human Umbilical Veins Endothelial Cells Homocysteine 3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyl tetrazolium bromide Nitric oxides Endothelial nitric oxide synthase |
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