不同牛奶消费量对雄性ICR小鼠生殖系统的影响

目的研究不同牛奶消费量对雄性ICR小鼠生殖器官的影响。方法取ICR小鼠(21d)96只,雌雄各半。随机将实验动物分为4组:5ml牛奶组,10ml牛奶组,15ml牛奶组,对照组。小鼠在给予牛奶12周后进行组内雌雄1∶1同笼配对繁殖,同笼6d后处死亲代雄鼠,待子代小鼠断奶后处死亲代雌鼠。喂饲期观察ICR小鼠一般情况、体重、饲料摄入量、交配率、怀孕率,喂饲结束后处死小鼠,观察以下指标:精囊腺、附睾、睾丸的湿重与组织学观察,精子计数,放射免疫法检测血清中雌二醇、雌三醇、睾酮浓度。结果雄性ICR小鼠生殖器官未发现明显的增重或减重,生育能力及病理学观察也未见异常。结论牛奶对雄性ICR小鼠生殖系统无明显影响。

Effect of different consumption of milk on the reproductive system of male ICR mice

Objective To investigate the Effect of different consumption of milk on the reproductive organs of male ICR mice. Methods 96 ICR mice （21 days age） ,with males and females in half, were randomly divided into 4 groups：5ml milk group, 10ml milk group, 15ml milk group and control group. After 12 weeks＇ milk feeding, the mice in same group were caged with the ratio of male and female ： 1： 1, after 6 - day breeding, the parental male mice were executed, then the parental female mice were executed after the descendant mice weaned. During the feeding, the mice＇ s general condition, body weight, feed intaking amount, mating rate and pregnancy rate were observed. After the feeding ended, the descendant mice were executed, and the wet weight and histological observation of seminal vesicle, epididymis, testicular were observed, sperm amount calculating, and the serum concentrations of estradiol, estriol ,and testosterone were detected by radio immunity method. Results There was neither significant weight gain or lose in reproductive organs of the male ICR mice, nor abnormality in the fertility and pathology observation. Conclusion The different consumption of milk has no obvious effect on the reproductive system of male ICR mice.