| TA29-Barnase 转基因雄性不育芥菜的获得 |
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| 引用本文： | 王倩,杨海鹏,邹敏,宋洪元.TA29-Barnase 转基因雄性不育芥菜的获得[J].中国蔬菜,2013,1(4):26-31. |
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| 作者姓名： | 王倩 杨海鹏 邹敏 宋洪元 |
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| 作者单位： | 西南大学园艺园林学院，南方山地园艺学教育部重点实验室，重庆 400715 |
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| 基金项目： | 重庆市自然科学基金项目(CSTC2011jjA80014);国家自然科学基金项目(30771462) |
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| 摘 要： | 将烟草TA29 花药绒毡层特异启动子驱动下的核糖核酸酶Barnase 基因，利用农杆菌介导的
方法导入渝丰榨菜幼苗下胚轴中，获得了彻底败育的雄性不育植株。细胞学观察结果显示：转基因芥菜植
株的花药绒毡层细胞提前降解，花粉母细胞退化，成熟花药中无正常花粉粒产生。转基因芥菜植株除了花
药瘦小、开花后无花粉散出、自交不能正常结籽外，还出现了花朵变小、雌蕊变短、种荚变短、结籽量下
降的现象。
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| 关 键 词： | 芥菜 雄性不育 TA29 启动子 Barnase 基因 |
| 收稿时间： | 2012-08-18; |
Procurement of Transgenic Male Sterile Mustard Plants Induced by TA29-Barnase Transformation |
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| WANG Qian,YANG Hai-peng,ZOU Min,SONG Hong-yuan.Procurement of Transgenic Male Sterile Mustard Plants Induced by TA29-Barnase Transformation[J].China Vegetables,2013,1(4):26-31. |
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| Authors: | WANG Qian YANG Hai-peng ZOU Min SONG Hong-yuan |
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| Affiliation: | College of Horticulture and Landscape Architecture， Key Laboratory of Horticulture Science for Southern Mountainous;
Regions， Ministry of Education， Southwest University， Chongqing 400715， China |
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| Abstract: | Ribonuclease Barnase gene was driven by tobacco TA29 anther tapetum specific
promoter，using the method of Agrobacterium mediated import in‘ Yufeng’ mustard〔 Brassica juncea
（L.）Czern. et Coss.〕seedling．Then a completely male sterile plant was gotten．The results of
cytological observation indicated a series of phenomenas showed that the ahead of schedule degradation
of anther tapetum cells in the TA29-Barnase transgenic plants，the degeneration of microspore mother
cells，and no normal pollen grains in matured anthers．The transgenic mustard plants had thin anther，
after flowering no pollen shed out，and normal seed could not produce when inbred， and also the flowers
became smaller，pistils shorter，seed pod shorter and the seed qu antity declined． |
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| Keywords: | Male sterility TA29-promoter TA29-promoter Barnase gene Barnase gene |
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