| 大肠杆菌的产毒能力与血清型关系——Ⅰ 被动免疫溶血试验(PIH)检测产肠毒素大肠杆菌不耐热肠毒素 |
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| 引用本文： | 王克智,陈艺林,汤秀兰,王群.大肠杆菌的产毒能力与血清型关系——Ⅰ 被动免疫溶血试验(PIH)检测产肠毒素大肠杆菌不耐热肠毒素[J].蚌埠医学院学报,1991(3). |
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| 作者姓名： | 王克智 陈艺林 汤秀兰 王群 |
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| 作者单位： | 蚌埠医学院检验系临床微生物学免疫学教研室
(王克智，陈艺林，汤秀兰)，蚌埠医学院检验系临床微生物学免疫学教研室(王群) |
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| 摘 要： | 本文自腹泻患儿粪便中分离155株大肠杆菌,经用PIH和Biken's两种方法对产肠毒素(LT)大肠杆菌的检测,检出率分别为8.3％和6.4％,远低于国外有关报道。实验用9株LT~+大肠杆菌的参考菌株经两种方法检测全部阳性。15株非产肠毒素肠道细菌,LT毒素检测均为阴性。PIH方法检测LT~+ETEC,在特异性和敏感性方面,均相当于放射免疫和基因探针技术,但更快速简便。
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| 关 键 词： | 产肠毒素大肠杆菌 被动免疫溶血 不耐热肠毒索 |
DETECTION OF HEAT LABILE ENTEROTOXIN OF E. COLI WITH PASSIVE IMMUNE HEMOLYSIS TECHNIQUE |
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| Wang Kezhi,Chen Yilin,Tan Xiulan,Wang Qun.DETECTION OF HEAT LABILE ENTEROTOXIN OF E. COLI WITH PASSIVE IMMUNE HEMOLYSIS TECHNIQUE[J].Journal of Bengbu Medical College,1991(3). |
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| Authors: | Wang Kezhi Chen Yilin Tan Xiulan Wang Qun |
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| Abstract: | This paper reports the detection of 155 strains E. coli from children with acute diarrhea. PIH assay was compared with Biken's test to identify LT+ETEC. The results obtained were 8.3% and 6.4% respectively, which were much lower than those reported abroad. The 9 reference strains of LT+ETEC detected by both methods were all positive, whereas the 15 strains of non-enterotoxin producing en-teropathogens were negative.
The PIH assay showed specificity and sensitivity by comparison with gene hybridizable and RIA technique, and presented to be more was rapid and simpler in clinical practice. |
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| Keywords: | enterotoxigenic Escherichia coli passive immune hemolysis heat labile cnterotoxin |
| 本文献已被 CNKI 等数据库收录！ |
