光动力疗法治疗变应性鼻炎的实验性研究

目的 初步研究光动力疗法（PDT）对实验性变应性鼻炎（AR）的治疗作用.方法 随机将18只新西兰兔均分为实验组A、阴性对照组B、空白对照组C共3组,采用卵清蛋白（OVA）致敏A、B2组兔制作动物AR模型,A组予以PDT治疗,B、C2组予以自然光处理,观察治疗前后症状及体征的变化,在光镜和透射电镜下观察鼻黏膜的变化.结果 与B组相比,A组兔经PDT治疗后,与AR相关症状和体征明显改善,光镜及透射电镜观察显示,炎性细胞明显减少,黏膜结构逐渐恢复,21 d后,A组与C组相比喷嚏次数、抓鼻次数及鼻分泌量差异无统计学意义（P＞0.05）,光镜及透射电镜下观察黏膜结构表现基本相似.结论 PDT对实验性AR有一定的治疗作用,为PDT用于AR定点治疗的临床应用提供了实验基础.

Experimental study on the treatment of allergic rhinitis with photodynamic therapy

Objective To preliminarily investigate the therapeutic effect of photodynamic therapy （PT） treating allergic rhinitis （AR）.Methods Eighteen New Zealand rabbits were randomly divided into 3 groups,experimental group A,negative control group B and blank control group C.Rabbits of A and B groups were sensitized with ovalbumin （OVA） to get animal AR model.Group A was treated with PDT while group B and C were applied with natural light treatment.Symptoms and signs before and after treatment were compared and the change of nasal mucosa was observed with light microscope and transmission electron microscope.Results After PDT treatment,group A rabbits＇ AR symptoms and signs were improved significantly compared to group B,light microscope and transmission electron microscope observation showed that the number of inflammatory cells of group A rabbits decreased significantly and the mucosal structure returned to normal after PDT treatment.After 21 d,as compared with group C,the differences of the times of sneezing and scratching and nasal secretion had no statistical significance （P＞0.05）.Conclusions PDT has certain therapeutic effect in experimental AR treatment,which can be used in AR treatment after consummating its therapeutic mechanism.