脉冲场凝胶电泳在白色假丝酵母菌基因核型分析中的应用

目的应用脉冲场凝胶电泳(PFGE)方法研究白色假丝酵母菌DNA核型,评价其作为分子流行病学监测手段的价值。方法从11例患者中分离出白色假丝酵母菌14株,采用PFGE法进行基因核型测定,MircroScan WalkAway96 RY-ID快速生化鉴定板进行菌种鉴定。结果14株白色假丝酵母菌有9种不同基因核型。其中从同一例患者不同部位标本中分离出的4株白色假丝酵母菌基因核型完全一致,表明其来源于同一菌株的不同克隆亚型;来源于不同患者的其他10株菌中有3株具有完全相同的基因核型,而其余7株菌具有不同的基因核型。结论PFGE可很好地显示出不同来源的白色假丝酵母菌菌株间的同源性及多态性,可作为其分子流行病学研究的有效手段。

Application of pulsed field gel electrophoresis on the karyotype analysis of Candida albicans

Objective To investigate the karyotype of Candida albicans DNA by pulsed field gel electrophoresis (PFGE), and evaluate the significance of the karyotype used as a method for molecular epidemiology controlling. Methods 14 isolates of C. albicans from various clinical samples were evaluated for molecular relatedness by PFGE. Isolates were identified by MircroScanWalkAway 96 RY-ID. Results The karyotype analysis revealed a total of 9 different genotypes among 14 isolates of C. albicans from 11 patients. Among the isolates, 4 were isolated from difference parts of a patient and had same karyotype, implying that they were from the same isolate and differed to different sub-clones. In another 10 isolates from different patients, 3 had same karyotype, and the karyotypes of the remainder 7 isolates were different. Conclusion PFGE may well reveal the autoploidy and polymorphism of the isolates of C. albicans with different origin, thus may be used as an effective method for molecular epidemiological study.