离子液体/盐双水相萃取分离锁阳中熊果酸

利用1-丁基-3-甲基咪唑氯([Bmim]Cl)和磷酸氢二钾(KH2PO4)双水相体系萃取分离锁阳中熊果酸,首先对[Bmim]Cl质量分数、KH2PO4质量分数、熊果酸粗提取液质量分数、NaCl质量分数、pH进行单因素研究,确定双水相体系组成为质量分数25%[Bmim]Cl-21%KH2PO4。然后,利用Box-Benhnken Design实验,对黄酮粗提取液质量分数、pH、NaCl质量分数进行了优化,结果表明:粗提液质量分数8.5%、NaCl质量分数1%、pH=4.7,熊果酸萃取率可达97.85%,与预测值98.3%接近。说明该优化工艺具有可行性。

Separation ursolic acid in Cynomorium songaricum Rupr by

In this study the potential use of ionic liquid-based aqueous two phase system (ILATPS) for the extraction of ursolic acid from Cynomorium songaricum Rupr was evaluated and optimized. The effects of independent variables(the mass fraction of [Bmim]Cl and KH2PO4, mass fraction of crude extract, mass fraction of NaCl and pH)were evaluated by single factor analysis. The optimization ATPS of 25%[Bmim]Cl-21%KH2PO4were selected based on their higher upper phase partitioning coefficient and the recovery .Using the optimized ILATPS solvent, the Box-Benhnken Design results showed mass crude extract 8.5%,NaCl 1%, pH4.7 were the optimal ILATPS extraction conditions. Maximum yield of extracted ursolic acid from the experiments was determined to be 97.85% under the optimal conditions, which was in good agreement with the predicted one of 98.3%. In conclusion, the optimized process has good reliability.