电化学生物传感器在有机磷农药分析中的应用

农业生产中有机磷农药的大量使用导致农产品中农药残留问题,对人类健康产生了严重的危害。因此,建立快速、灵敏的有机磷农药残留分析方法,具有重要的研究意义。近年来,电化学生物传感器因具有灵敏性高、选择性好、制备成本低、操作简单、携带方便、抗干扰性强等优点而被广泛用于农药残留分析,成为食品安全快速检测技术重要的发展方向之一。本文主要综述了目前国内外检测有机磷农药的电化学生物传感方法。简要介绍了生物传感器的概念、检测原理,重点讨论了电化学酶生物传感器(乙酰胆碱酯酶、丁酰胆碱酯酶、磷酸水解酶)及电化学免疫生物传感器在检测有机磷农药分析中的应用,并对各类传感器的检测原理、最新进展及存在的优、缺点进行了阐述,最后对电化学生物传感器存在问题的解决途径及未来发展方向进行了展望。

Application of electrochemical biosensors for the detection of organophos-phorus pesticide

The excessive use of organophosphorus pesticide (OPs) in the process of agricultural production resulted in OPs residues in the agricultural products, which had been found to seriously threaten human health. Hence, it is of great significance to establish rapid and sensitive methods for analysis of OPs residues. In recent years, electrochemical biosensors have been widely used in the detection of OPs residues with the merits of high sensitivity, selectivity, low-cost, easy operation, portability and excellent capacity of resisting disturbance, and they are considered as an important development trend of rapid detection technology for food safety. This paper focused on the current development of electrochemical biosensing methods for OPs residues detection at home and abroad. This article briefly introduced the concept, working principle of the biosensor, and then highlighted the applications of electrochemical enzyme biosensors (acetylcholine esterase, butyrylcholine esterase and organophosphorus hydrolase) and electrochemical immuno biosensors in analyzing the OPs as well as the sensing mechanism, recent progresses, the merits and demerits of the individual biosensor. Finally, the potential resolutions to the existing problems and the future development of the electrochemical biosensor were prospected.