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芝麻香型白酒堆积过程微生物群落结构特征的PLFA分析
引用本文:徐泽江,孟镇,钟其顶,熊正河,盛清. 芝麻香型白酒堆积过程微生物群落结构特征的PLFA分析[J]. 食品与发酵工业, 2012, 38(4): 20-24
作者姓名:徐泽江  孟镇  钟其顶  熊正河  盛清
作者单位:1. 浙江理工大学生命科学学院,浙江杭州310018/中国食品发酵工业研究院,北京100027
2. 中国食品发酵工业研究院,北京,100027
3. 浙江理工大学生命科学学院,浙江杭州,310018
基金项目:第三批济南“泉城学者”建设工程中标项目“芝麻香型白酒生产工艺研发”项目;2009质检公益项目“葡萄酒和白酒行业规范化技术标准基础研究”(200910220)
摘    要:应用磷脂脂肪酸分析方法(PLFA)研究了芝麻香型白酒堆积过程微生物生物量和群落结构的变化。结果表明:在整个堆积过程,PLFA的种类和数量呈现一定的变化规律,可以反应微生物群落结构的变化。堆积过程中的优势PLFA是直链饱和脂肪酸和偶数碳不饱和脂肪酸。堆积的时间和温度对生物量的含量影响显著。同时,细菌是整个堆积过程的优势微生物菌群,革兰氏阴性菌是细菌中的优势菌群。而厌氧菌和好氧菌在不同的样品中差异较大。

关 键 词:芝麻香型白酒  磷脂脂肪酸  微生物群落结构  微生物生物量

Microbial Community Changes During Stacking Fermentation in the Production of Sesame-flavor Liquor Using PLFA
Xu Ze-jiang,Meng Zhen,Zhong Qi-ding,Xiong Zheng-he,Sheng Qing. Microbial Community Changes During Stacking Fermentation in the Production of Sesame-flavor Liquor Using PLFA[J]. Food and Fermentation Industries, 2012, 38(4): 20-24
Authors:Xu Ze-jiang  Meng Zhen  Zhong Qi-ding  Xiong Zheng-he  Sheng Qing
Affiliation:1(School of Life Science,Zhejiang Sci-Tech University,Hangzhou 310018,China) 2(China National Research Institute of Food & Fermentation Industries,Beijing 100027,China)
Abstract:Microbial biomass and community structure were investigated during stacking fermentation in the production of Sesame-flavor Liquor using the phospholipid fatty acid technique.The results indicated that the types and number of microorganisms PLFAs shows certain variation rules,and that can be a reflection of changes in microbial community structure.Straight chain saturated fatty acids and even-carbon unsaturated fatty acids were the predominant PLFAs.The impact of stack temperature and time on microbial biomass was obvious.Besides,the predominant microorganism is bacteria and G~-is the predominant bacteria during stacking fermentation.The difference between anaerobic and aerobic bacteria is quite visible in different samples.
Keywords:sesame-flavor liquor  phospholipid fatty acid(PLFA)  microbial community structure  microbial biomass
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