小鼠脂肪干细胞的免疫原性及移植安全性

背景:多组实验证实脂肪干细胞体外能够大量扩增,经一定诱导条件作用后能够向3个胚层分化,并且能够加以一定的基因修饰.目的:观察小鼠脂肪干细胞的免疫原性和经静脉移植后的安全性.方法:体外分离培养小鼠脂肪干细胞,流式细胞仪鉴定细胞表面的免疫标记物;体外淋巴细胞增殖试验检测脂肪干细胞对淋巴细胞的活化能力;经小鼠尾静脉移植脂肪干细胞,观察细胞在体内对小鼠T、B淋巴细胞的刺激作用;仔细解剖移植后小鼠,观察细胞移植后的成瘤性.结果与结论:脂肪干细胞在体外容易扩增培养,表达间质干细胞表面标记物;体外与淋巴细胞共培养时,不能有效刺激淋巴细胞增殖;经静脉移植后,不能激活机体细胞免疫反应,但是能够活化B淋巴细胞,激活机体体液免疫反应;细胞移植后未观察到肿瘤形成和组织畸变.提示脂肪干细胞总体免疫原性较低,移植后不会激活宿主细胞免疫系统;脂肪干细胞移植后无肿瘤样生长,安全性较好.

Immunogenicity and safety of mice adipose-derived stem cells

BACKGROUND: Multiple sets of experiments confirmed that adipose-derived stem cells (ADSCs) can amplification in vitro, and can differentiated to three germinal layers under certain conditions and can be modified with gene. OBJECTIVE: To investigate the immunogenicity and safety of mice ADSCs after vein transplantation. METHODS: ADSCs were isolated from adipose tissue of mice; immunity markers of these cells were observed by flow cytometry. In vitro lymphocyte hyperplasy experiment was used to determine the effect of ADSCs on activated ability of lymphocyte. ADSCs were transplanted into mice model through caudal vein and observed the stimulatory function of ADSCs on T and B lymphocyte in vivo. Anatomy the mice model after transplantation and detect the tumor of the transplanted ADSCs. RESULTS AND CONCLUSION: ADSCs could culture in vitro and express the immunity markers of mesenchymal stem cells. When co-culture with lymphocyte, ADSCs could not stimulate the proliferation of lymphocyte efficiency. After transplantation through caudal vein, ADSCs could not stimulate immunological reaction of lymphocyte, but ADSCs could activate B lymphocyte and the humoral immunity. No tumor formation and tissue distortion were observed after transplantation. ADSCs have low immunogenicity, and could not stimulate the immune system of host cells and had a good safety.