维生素E对大剂量维生素D3所致心肌损伤保护作用的实验性研究

目的：观察大剂量VD3对心肌的损伤作用及维生素E（VE）对其保护机制。方法：Wistar大鼠36只随机分为3组，VE＋VD3组经口灌胃VE油剂8．3mg／（kg·d），共7次，后3次给药加VD3油剂15万IU／只。VD3组同法给予生理盐水和VD3，对照组给予生理盐水。测定血清、心肌中的钙含量，血清中VE含量，测量血清、心肌和肝脏中谷胱甘肽过氧化物酶（GSH—Px）和脂质过氧化物酶（丙二醛，MDA）含量。测量心肌损伤面积。结果：VD3组血清中VE含量，血清、心肌和肝脏中GSH—Px活性明显低于VE＋VD,组和对照组（P〈0．01），VE＋VD3组略高于对照组，但无显著性差异（P〉0．05）。VD3组血清、心肌、肝脏组织中MDA，血清、心肌钙水平明显高于VE＋VD3组和对照组（P〈0．01），VE＋VD3组与对照组相比差异不显著（P〉0．05）。VE＋VD3组心肌坏死面积明显小于VD3组（P〈0．05）。结论：预先给予天然VE能保护大剂量VD3所致大鼠心肌损伤。

Experimental Research on Protective Effect of Vitamin E on Myocardial Injury Induced by High Dosage of Vitamin D_3

Objective： To evaluate the effect of Vitamin E （VE） on myocardial injury induced by high dosage of Vitamin D3 （VD3） in rats. Methods： Thirty-six Wistar rats were randomly divided into three groups. Group VE ＋ VD3 was given VE 8. 3 mg/（kg·d） for 7 days and in additon, VD3 150000 IU/d during the last three days by intragastric administration once per day. Group VD3 was given normal saline and the same dosage of VD3 as group VE ＋ VD3. Control group was given normal saline. Contents of VE and calcium in serttm and myoeardium were observed. The activity of glutathione-peroxidase（GSH-Px） and content of Malondialdehyde （MDA） were observed. Results： Contents of VE and activity of GSH-Px were decreased significantly in group VD3 than those in group VE ＋ VD3 and control group （P〈0. 01）. Content of MDA and calcium were increased significantly in group VD3 than those in group VE ＋ VD3 and control group （P〈0. 01）, and there was no significance between group VE ＋ VD3 and control group （P〉0. 05）. The area of myocardial injury was obviously smaller in group VE ＋ VD3 than that in group VD3 （P〈0. 05）. Conclusion： VE can protect myocardial injury in rats induced by high dosage of Vitamin D3.