| 蛋白质的侧基修饰方法研究 |
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| 引用本文： | 夏淳淳,罗朝阳,范浩军,张琪. 蛋白质的侧基修饰方法研究[J]. 皮革科学与工程, 2011, 21(2) |
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| 作者姓名： | 夏淳淳 罗朝阳 范浩军 张琪 |
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| 作者单位： | 1. 四川大学制革清洁技术国家工程实验室,四川,成都,610065
2. 四川大学轻纺与食品学院,四川,成都,610065 |
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| 基金项目： | 国家自然科学基金资助项目(20976110) |
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| 摘 要： | 以明胶作为皮胶原模拟物,以甲醇、二碳酸二叔丁酯((Boc)2O)作为封端剂分别封闭明胶的羧基、氨基,并用FT-IR、等电点(PI)和总碳含量(TOC)等对未改性明胶、羧基封闭明胶、氨基封闭明胶分别进行结构和性能表征。结果显示,甲醇、(Boc)2O与明胶反应后分别有酯键、酰胺键生成;羧基封闭的明胶等电点由未改性明胶的4.8上升到6.2,而氨基封闭的明胶等电点则下降到3.6;未改性明胶、羧基封闭明胶、氨基封闭明胶单位体积的总C含量分别是41.48、46.46、48.96 mg/L,羧基封闭明胶和氨基封闭明胶总有机碳含量都高于未改性明胶,表明明胶的羧基、氨基分别被封闭。
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| 关 键 词： | 蛋白质 封端剂 侧基封闭 结构表征 |
The Modification Methods of Protein |
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| XIA Chun-chun,LUO Zhao-yang,FAN Hao-jun,ZHANG Qi. The Modification Methods of Protein[J]. Leather Science and Engineering, 2011, 21(2) |
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| Authors: | XIA Chun-chun LUO Zhao-yang FAN Hao-jun ZHANG Qi |
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| Affiliation: | XIA Chun-chun1,LUO Zhao-yang1,FAN Hao-jun1,ZHANG Qi2(1.National Engineering Laboratory for Clean Technology of Leather Manufacture,Sichuan University,Chengdu 610065,China,2.College of Light Industry,Textile and Food Engineering,China) |
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| Abstract: | Gelatin was used as a model of collagen,methanol and(Boc)2O were used as termination agents of carboxyl and amino,respectively,the termination reaction of carboxyl and amino of gelatin were investigated and the difference in structure and properties of gelatin(before and after modification)were characterized with FT-IR,PI and TOC analysis.Results showed that new-COOR and-CONH-bonds were generated after terminated with methanol and(Boc)2O respectively,the isoelectric point of carboxyl terminated gelatin rise... |
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| Keywords: | protein termination agents modified structural performance |
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