血管紧张素转换酶基因多态性与脑梗死危险因素的关系

目的：探讨血管紧张素转换酶（ACE）基因多态性与中国汉族人脑梗死危险因素关系。方法：应用聚合酶链反应（PCR），测定165例脑梗死、101例高血压患者和106例正常对照者ACE基因插入／缺失（I／D）多态性，用比色法测定血清ACE水平，并调查脑梗死经典的危险因素，结果：脑梗死组DD型基因频率为0．43，高于高血压组的0．31(X^2=4．03，P＜0．05）和正常对照组的0．17（X^2=19．86，P＜0．01），且D等位基因亦明显高于高血压组和正常对照组（X^2=18．30，12．41、29．00、12．10，P＜0．01）。脑梗死组血清ACE水平明显高于正常对照组（F＝2240．06，P＜0．01）其中DD基因型血清ACE水平又高于同组DI基因和Ⅱ基因（F＝8．83，P＜0．01）。结论：ACE基因缺失多态性可能是中国人汉族脑梗死独立危险因素，循环ACE活性与基因缺失多态性相关。

The relationship between angiotensin converting enzyme gene polymorphism and risk factors for cerebral infarct

OBJECTIVE: To explore the relationship between angiotensin converting enzyme (ACE) gene polymorphism and risk factors of cerebral infarct (CI) in Chinese Han nationality. METHODS: One hundred and sixty-five cases with cerebral infarct, 101 cases of essential hypertension patients with 106 normal persons in Chinese Han serving as controls were detected using polymorase chain reaction (PCR) and genetic methods. ACE contents in serum were measured by colorimetric method. Risk factors of cerebral infarct were assessed by standard questionnaire, physical examination and blood tests. RESULTS: ACE DD genotype appeared more common in patients of cerebral infarct when comparing with essential hypertension groups (0.43 versus 0.31, chi 2 = 4.03, P < 0.05) and normal controls (0.43 versus 0.17, chi 2 = 19.86, P < 0.01). D:I allele frequency appeared to be 0.66:0.34 in cerebral infarct patients and 0.41:0.59 in controls (chi 2 = 32.85, P < 0.01). In basal ganglia infarct and thalamus infarct groups, ACE DD genotype and allele ratio distribution were remarkably different to that in normal subjects (chi 2 = 18.30, 12.41, P < 0.01). The mean levels of serum in cerebral and hypertension were higher than in normal controls (F = 2,240.06, P < 0.01), and serum ACE activity in ACE DD genotype was significantly higher than that in ACE DI and II genotype in cerebral group (F = 8.83, P < 0.01). CONCLUSIONS: The ACE gene deletion polymorphism might serve as an independent risk factor for cerebral infarct in Chinese Han nationality while circulation ACE activity might be related to gene deletion polymorphism.