8例B细胞淋巴瘤的T细胞基因CD4 V4突变及其相关蛋白的表达

【目的】检测B细胞淋巴瘤组织中T细胞的CD4V4基因突变情况及其相应病例的Fas/FasL、CD4、bcl 2和CyclinD1蛋白表达特征。【方法】PCR SSCP技术筛选检测 5 6例B细胞淋巴瘤CD4分子的V4外显子突变状况。用免疫组化方法检测有突变的B细胞淋巴瘤组织中L2 6 ,UCHL1,CD3,Fas ,FasL ,bcl 2和CyclinD1蛋白表达情况。【结果】 8例 / 5 6例(14 2 % )T细胞CD4V4外显子有变异。其中小淋巴细胞性淋巴瘤 (smalllymphocytelymphoma,SLL)和淋巴母细胞性淋巴瘤(lymphoblasticlymphoma ,LBL)的Fas,FasL和CD4蛋白均为弱或无表达 ;滤泡性淋巴瘤 (follicularlymphoma ,FL) 3项均强表达 ;3例弥漫性大B细胞淋巴瘤 (diffuselargeBcelllymphoma ,DLBL)和 1例套细胞淋巴瘤 (mantlecelllymphoma ,MCL)Fas和FasL强表达 ,CD4无或弱表达。Fas和FasL双强表达的 4例 ,均有bcl 2和 /或CyclinD1过表达。【结论】B细胞淋巴瘤中CD4V4基因有突变 ,并提示这种突变与细胞死亡和细胞周期调控异常有关。

CD4 V4 Mutation of T Cells and Its Ralative Protein Expression in 8 Cases of B Cell lymphoma

Objective Detection of mutation in CD4 V4 exon and expression of proteins associated with Fas, FasL, bcl-2,CD4 and CyclinD1 in B cell lymphoma. Methods The exon V4 mutation of CD4 gene was examined on 56 cases of B cell lymphoma specimens by means of PCR-SSCP and the expression of proteins of CD3,CD4, Fas, FasL, bcl-2 and Cyclin D1 by immunohistochemistry. Results The V4 mutaion rate of CD4 gene was 14 2% (8/56). Among them, CD4, Fas, FasL expressed weak or none in both SLL and LBL; on the contrary, FL was strong; 3/4 cases of DLBL and 1 case MCL expressed both Fas and FasL strong, but CD4 not expressed. Occasionally, they express bcl-2 and /or Cyclin D1 strong too. Conclusion There are CD4 V4 mutaion in B cell lymphoma and abnormal regulators of cell cycle and death.