针刺对女性生殖内分泌影响的实验研究

目的 :观察针刺对外周血中FSH、LH、E2 、T、P的影响。方法 :选健康育龄女性 2 8名 ,以耳穴与体穴、手法和电针相结合的方法进行治疗 ,采用自身对照 ,检测治疗前后各项指标。结果 :针刺前后FSH、LH的变化无明显差异 (P >0 .0 5) ,而E2 、T、P的变化具有显著性差异 (P <0 .0 5)。结论 :针刺对垂体促性腺激素的分泌无明显影响 ,对卵巢性激素的分泌具有明显的抑制性效应。

Experimental Study on the Effect of Acupuncture on Genital Endocrine in Healthy Women of Childbearing Age

Objective: To observe the effect of acupuncture on pituitary gonadotropic hormones and ovariogenic hormones in the peripheral blood in healthy women of childbearing age. Methods: 60 questionnaires on basal body temperature were given to 60 females at the age of 22～35 years for observing the variation of basal body temperature and menstrual cycle within 2 menstruation periods, and 28 volunteer females with regular menstruation and basal body temperature presenting typical ovulation were selected as the subjects in this study.Before acupuncture treatment, blood samples were taken and kept in the fridge one day before and on the day of the calculated ovulation. These 28 subjects accepted acupuncture stimulation during the forth menstruation period, beginning from the first 3 days on before ovulation, once daily continuously for 4 days, followed by taking blood samples. Auricular and body acupoints used were G 4 Area (both sides), Shimen(CV 5) and Sanyinjiao (SP 6,one side). After inserting needles into bilateral G 4, rotated the needles for 1 min, then punctured the body acupoints till obtaining needle sensations, afterwards twisted and thrust the needles for 1 min. Then, Shimen (CV 5) and Sanyinjiao(SP 6) were stimulated electrically for 15 min with an Electroacupuncture Therapeutic Apparatus. Results: Results indicated that before and after acupuncture, the concentration values of follicle stimulating hormone (FSH) and luteinizing hormone (LH) in the peripheral blood were 3.90±2.30 IU/L and 11.54±11.73 IU/L, 3.38±1.76 IU/L and 8.68±8.15 IU/L respectively, no significant differences were found between pre and post acupuncture ( P >0.05), meaning no effect of acupuncture on pituitary gonadotropic hormones. Before and after acupuncture, the concentrations of blood estradiol (E 2), testosterone (T) and pregnendione (P) were 0.54± 0.42 nmol/L, 6.84±2.88 nmol/L and 3.86±2.76 ng/L, 0.35±0.21 nmol/L, 5.03±2.16 nmol/L and 2.97±2.05 ng/mL separately, showing considerable differences between pre and post acupuncture in each index ( P <0.01, 0.001 and 0.05). It displays that acupuncture possesses an inhibitory effect on the secretion activities of ovariogenic hormones.Conclusion: Acupuncture stimulation has a suppressing effect on blood E 2, T and P in healthy women of childbearing age.