| Gonadotrophin-releasing hormone-Ⅰ and -Ⅱ stimulate steroidogenesis in prepubertal murine Leydig cells in vitro |
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| 引用本文： | Lin YM,Liu MY,Poon SL,Leu SF,Huang BM. Gonadotrophin-releasing hormone-Ⅰ and -Ⅱ stimulate steroidogenesis in prepubertal murine Leydig cells in vitro[J]. Asian journal of andrology, 2008, 10(6): 929-936 |
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| 作者姓名： | Lin YM Liu MY Poon SL Leu SF Huang BM |
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| 作者单位： | [1]Department of Urology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan, China; [2]Department of Environmental and Occupational Health, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan, China; [3]Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan, China; [4]Institute of Bioindustrial Technology, College of Human Ecology, HungKuang University, Taichung 43302, Taiwan, China |
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| 摘 要： | Aim： To study the effect and mechanism of gonadotrophin-releasing hormone （GnRH） on murine Leydig cell steroidogenesis. Methods： Purified murine Leydig cells were treated with GnRH-Ⅰ and -Ⅱ agonists, and testosterone production and steroidogenic enzyme expressions were determined. Results： GnRH-Ⅰ and -Ⅱ agonists significantly stimulated murine Leydig cell steroidogenesis 60%-80% in a dose- and time-dependent manner （P 〈 0.05）. The mRNA expressions of steroidogenic acute regulatory （STAR） protein, P450scc, 3β-hydroxysteroid dehydrogenase （HSD）, but not 17β-hydroxylase or 17β-HSD, were significantly stimulated by both GnRH agonists with a 1.5- to 3-fold increase （P 〈 0.05）. However, only 3β-HSD protein expression was induced by both GnRH agonists, with a 1.6- to 2-fold increase （P 〈 0.05）. Conclusion： GnRH directly stimulated murine Leydig cell steroidogenesis by activating 3β-HSD enzyme expression.
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| 关 键 词： | 促性腺激素释放 青春期 干细胞 症状 |
Gonadotrophin-releasing hormone-I and -II stimulate steroidogenesis in prepubertal murine Leydig cells in vitro |
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| Lin Yung-Ming,Liu Ming-Yie,Poon Song-Ling,Leu Sew-Fen,Huang Bu-Miin. Gonadotrophin-releasing hormone-I and -II stimulate steroidogenesis in prepubertal murine Leydig cells in vitro[J]. Asian journal of andrology, 2008, 10(6): 929-936 |
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| Authors: | Lin Yung-Ming Liu Ming-Yie Poon Song-Ling Leu Sew-Fen Huang Bu-Miin |
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| Affiliation: | Department of Urology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan, China. |
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| Abstract: | gonadotrophin-releasing hormone Leydig cells murine steroidogenesis stimulation |
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| Keywords: | gonadotrophin-releasing hormone Leydig cells murine steroidogenesis stimulation |
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