| 重组酵母测评系统对环境样品雌激素活性的检测 |
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| 引用本文： | 王娟,张遵真,张浩,李娜,车望军.重组酵母测评系统对环境样品雌激素活性的检测[J].环境科学学报,2005,25(12):1698-1701. |
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| 作者姓名： | 王娟 张遵真 张浩 李娜 车望军 |
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| 作者单位： | 四川大学华西公共卫生学院环境卫生教研室,成都,610041 |
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| 摘 要： | 采用重组酵母测评系统对香烟烟雾溶液和汽车尾气提取物分别进行雌激素活性的测定.结果显示在受试浓度下,PBS烟雾溶液和DMSO烟雾溶液的β-半乳糖苷酶活性与溶剂对照相比均无显著性差异(P>0·05).汽油燃料车尾气在600mL·mL-1时,其酵母菌液OD600明显低于DMSO对照组(P<0·05);在150mL·mL-1剂量时,汽油燃料车尾气所检出的β-半乳糖苷酶活性为44·56,与DMSO对照组相比有显著性差异(P<0·05),当剂量增加至300mL·mL-1,酶活性升高为66·43,约为对照组的12倍,其它各剂量组酶活性与DMSO对照组相比无显著性差异(P>0·05);甲醇燃料车尾气的β-半乳糖苷酶活性与溶剂对照相比无显著性差异(P>0·05).提示汽油燃料车尾气具有弱雌激素活性,甲醇燃料车尾气和香烟烟雾溶液未检出雌激素活性.
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| 关 键 词： | 重组酵母测评系统 香烟烟雾 汽车尾气 雌激素活性 |
| 文章编号： | 0253-2468(2005)12-1698-04 |
| 收稿时间： | 05 31 2005 12:00AM |
| 修稿时间： | 09 26 2005 12:00AM |
Detection of estrogenic activity of environmental mixtures with recombinant yeast estrogen system |
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| WANG Juan,ZHANG Zunzhen,ZHANG Hao,LI Na and CHE Wangjun.Detection of estrogenic activity of environmental mixtures with recombinant yeast estrogen system[J].Acta Scientiae Circumstantiae,2005,25(12):1698-1701. |
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| Authors: | WANG Juan ZHANG Zunzhen ZHANG Hao LI Na and CHE Wangjun |
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| Affiliation: | Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041,Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041,Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041,Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041 and Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041 |
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| Abstract: | |
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| Keywords: | recombinant yeast estrogen system cigarette smoking solution vehicle exhaust extracts estrogen activity |
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