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不同产地栀子炮制品中绿原酸含量比较
引用本文:段启,陈华师. 不同产地栀子炮制品中绿原酸含量比较[J]. 河南中医药学刊, 2010, 0(2): 260-261
作者姓名:段启  陈华师
作者单位:[1]广东食品药品职业学院,广东广州510520 [2]广东康美药业股份有限公司,广东普宁515300
摘    要:目的:测定了不同产地栀子炮制品中绿原酸的含量。方法:采用外标法。Zorbax XDB-C18(4.6×150mm,5μm)色谱柱,乙腈-1.1%冰乙酸(0-12min:7:93;12-20min:10:90),流速为1.0ml/min,柱温25℃,检测波长为326nm。结果:测得10个产地栀子中生栀子、炒栀子、焦栀子中绿原酸含量随着炮制程度的深入,逐渐降低。结论:本研究建立的栀子中绿原酸含量测定方法简便易行,结果准确、可靠,便于对栀子不同炮制品中绿原酸含量进行检测,有利于栀子炮制品的质量控制。

关 键 词:栀子  焦栀子  绿原酸

Compare of Chlorogenic Acid in Gardeniae from Different Areas and in Various Prepared Products
Duan Qi,Chen Huashi. Compare of Chlorogenic Acid in Gardeniae from Different Areas and in Various Prepared Products[J]. Journal of Henan college of Traditional Chinese Medicine, 2010, 0(2): 260-261
Authors:Duan Qi  Chen Huashi
Affiliation:1 Guangdong Food and Drug Vocational College,Guangzhou,Guangdong,China 510520;2 Guangdong Kangmei Pharmaceutical Co.,Ltd,Puning,Guangdong,China 515300 )
Abstract:Objective:To develop a method using HPLC for determination content of Chlorogenic acid in Gardenia from different areas and in various prepared products.Methods:Zorbax XDB-C18(4.6 150 mm,5 μm),mobile phase:acetonitrile-1.1% acetic acid glacial((0-12 min:7:93;12-20 min:10:90)),detected wavelength:326 nm.The column temperature was maintained at 25℃.Results:In Gardenia from different areas and in various prepared products,chlorogenic acid content was gradually to decrease.Conclusion:The HPLC method is simply and suitable to control quantitative of Gardenia.Many facts show a influence on the contents of Chlorogenic acid in Gardenia from different areas and in various prepared products,it's good for quality control in Gardenia from prepared products.
Keywords:Fructus Gardeniae  Fructus Gardeniae(parching it into yellow)  Chlorogenic acid
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