| 电针12h对颅脑损伤大鼠脑组织[Ca（2＋）]i和NE含量影响的实验研究 |
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| 引用本文： | 黄长军,宋金玲,李志刚. 电针12h对颅脑损伤大鼠脑组织[Ca（2＋）]i和NE含量影响的实验研究[J]. 中医药学报, 2010, 38(2): 63-65 |
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| 作者姓名： | 黄长军 宋金玲 李志刚 |
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| 作者单位： | 1. 北京中医药大学,北京,100029
2. 北京中医药大学东方医院,北京,100029 |
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| 摘 要： | 目的：对电针12h对颅脑损伤大鼠脑组织[Ca2＋]i和NE含量的影响进行实验研究。方法：采用激光共聚焦显微镜的动态扫描功能和荧光分子探针技术,从针灸血清学和细胞学角度,对针刺通过颅脑损伤大鼠血清影响SY5Y细胞[Ca2＋]i进行研究;采用高效液相色谱法研究电针对颅脑损伤大鼠脑组织NE含量的影响。结果：各组血清孕育12h后SY5Y细胞[Ca2＋]i相比较显示,模型对照组的荧光强度明显高于空白对照组（P﹤0.01）,针刺治疗组的荧光强度明显低于模型对照组（P﹤0.05）;各组大鼠脑组织NE含量相比较,模型对照组显著高于空白对照组（P﹤0.01）,针刺治疗组明显低于模型对照组（P﹤0.05）。结论：电针12h可减慢颅脑损伤大鼠脑组织[Ca2＋]i和NE含量的上升趋势,提示电针可能是通过抑制脑皮质内大量NE聚集,降低皮质神经元的兴奋性,从而抑制细胞内Ca2＋滞留和超载,减轻脑水肿,对脑组织起到保护作用。
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| 关 键 词： | 电针 颅脑损伤 血清 SY5Y细胞 [Ca2＋]i NE |
Experimental Studies on the Effection of 12 Hours Electro -acupuncture in [Ca2+]i and NE Level of CCI rats |
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| HUANG Chang-jun,SONG Jin-ling,LI Zhi-gang. Experimental Studies on the Effection of 12 Hours Electro -acupuncture in [Ca2+]i and NE Level of CCI rats[J]. Acta Chinese Medicine and Pharmacology, 2010, 38(2): 63-65 |
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| Authors: | HUANG Chang-jun SONG Jin-ling LI Zhi-gang |
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| Affiliation: | HUANG Chang-jun1,SONG Jin-ling2,LI Zhi-gang1 (1. Beijing University of Chinese Medicine,Beijing 100029,China,2. Dongfang Hospital of BUCM Beijing,China) |
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| Abstract: | Objective:To study on the effection of 12 hours electro-acupuncture in [Ca2+ ]i and NE level of craniocerebral injury rats. Methods: From the ways of serology and cytology,Adopted Dynamic confocal laser scanning microscope and Fluorescent molecular probe techniques,to study on the influence of acupuncture in SY5Y cells[Ca2 + ]i by the serum of CCI rats. To study the influence of electro-acupuncture in NE level of CCI brain tissue by high performance liquid chromatography. Results: Gestated by the serum of C... |
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| Keywords: | [Ca2+]i NE |
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