大鼠脑缺血/再灌注后AQP4的表达与脑水肿的关系

目的: 探讨在大鼠脑缺血/再灌注脑水肿中水通道蛋白-4(AQP4)表达水平的动态变化与脑含水量之间的关系。 方法: 50只SD大鼠,随机分为假手术组和6,12,24,48 h脑缺血/再灌注模型组,Western blot法测定AQP4的表达,干湿重法测定脑含水量。 结果: 与假手术组比较,模型组大鼠缺血侧脑含水量和AQP4的表达水平随着时间的推移不断增加,均在24 h时达到最高峰;12~24 h时间段脑含水量显著增加(P<0.01),以及AQP4表达水平升高,48 h时两者均稍有下降。而健侧脑含水量和APQ4的表达水平各时间点之间差异不显著。采用Spearman相关性分析结果显示,脑含水量和AQP4表达水平相关性显著(P<0.05)。 结论: 大鼠脑缺血/再灌注后脑含水量和AQP4表达水平变化趋势基本一致,两者呈时相正相关性。

Relationship between AQP4 Expression and Brain Water Content after Cerebral Ischemic/Reperfussion in Rats

Objective: To investigate the relationship between aquaporin-4(AQP4) expression and brain water content in brain edema after cerebral ischemic/reperfussion(I/R) in rats. Method: Total 50 adult male Sprague-Dawley rats were randomly divided into sham group and 6,12,24,48 h cerebral ischemic/reperfussion groups. Brain water content was measured by the Wet-dry method and the expression of AQP4 was observed by Western blot. Result: Brain water content and APQ4 expression of ischemic side in model group was increased with delayed I/R period, and at 24 h the change was most marked, brain water content increased significantly and AQP4 expression increased from 12 h to 24 h,There was a slight decrease at 48 h. The difference of brain water content and APQ4 expression in contralateral side was not significant among different time points. The data were analyzed by method of Spearman, the results showed that the correlation was significant(P<0.05). Conclusion: The consistency is obvious between the change of AQP4 expression and brain edema in ischemic side after cerebral ischemic reperfusion.