电磁脉冲对海马C—FOS表达的影响

目的 :本研究拟通过观察电磁脉冲 (EMP)辐照大鼠后其海马C FOS表达的改变 ,初步探讨EMP致海马损伤的机制。方法 :用高场强电磁脉冲模拟源 (所产生的脉冲上升时间为 2 0ns ,脉宽为30 μs) ,在 2min内辐照大鼠 5次 ,并设对照组 ,辐照后于 1,6,12 ,2 4和 4 8h活杀动物 ,采用免疫组织化学SP方法显示C FOS的表达并在光镜 10× 4 0倍视野下 ,应用CMIAS Ⅱ图像分析仪对阳性细胞的图像学参数 积分光密度和平均光密度测定分析 ,最后结果经SPSS8.0统计软件统计分析。结果 :被辐照组大鼠在 6～ 12h时海马锥体细胞及齿状回颗粒细胞C FOS均呈高表达 ,积分光密度和平均光密度值在 12h时最大 ,且显著高于对照组 (P <0 .0 5和P <0 .0 1)。结论 :EMP可引起C FOS在海马锥体细胞及齿状回颗粒细胞的高表达 ,且C FOS可能在EMP致大鼠的脑损伤中起重要作用

Influence of EMP on the C-FOS expression in hippocampus

ObjectiveTo explore the possible injury mechani sm in brain of rat irradiated by EMP through observing the changes in expression of CFOS in hippocampus and dentate gyrus.MethodsThe group A, group B and group C were irradiated by high field intensity(6104,2104 a nd 6~{!A~}103 V/m respectively)electromagnetic puls es(EMP)for 5 times within 2 minutes respectively and no treatment was given to g roup D(control group).The rats were sacrificed at 1, 6, 12,24 and 48 h,sep arately. Immunohistochemical method was used to investigate the expression of C FOS and image analysis for CFOS p ositive cells was performed by CMTAS~{*2"r~} image analysis system at a magnificatio n of 400. Results The rats of irradiated groups had high expres sion of CFOS in neurons of hippocampus and dentate gyrus at 6-12 h, and the nu mber of CFOS positive cells was significantly increased as compared with contr ol group ~{*)~}(P<0.05)~{**~}.Conclusions The EMP could cause significant high CFOS expression in hippocampus and gyrus,which may pla y an important role in the injury of rat brain by EMP.