大黄中4种蒽醌类化合物抑幽门螺杆菌效果比较

 目的:对4种大黄蒽醌化合物抑制幽门螺杆菌( Helicobacter pylori )进行实验研究。方法:用梯度碱提取法，将大黄中的4种蒽醌类化合物提取，然后用试管稀释法和纸片扩散法，分别观察其对幽门螺杆菌的抑菌效果。结果:4种大黄蒽醌，最低抑菌浓度及抑制幽门螺杆菌环分别为:大黄素(emodin) 0.40μg· ml^{-1(30mm,强)、大黄酸(rhein)0.60μg·ml-1(28mm,强)、大黄酚(chrysophanol ) 0.78μg·ml-1(24mm,强)、芦荟大黄素(aloe-emodin)0.85μg·ml-1(23mm,强)。衬照品大黄提取物(Rheum officinale extracts)仅为17.24μg·ml-1(15mm，中)。空白对照为灭菌注射用水，无抑菌环，幽门螺杆菌生长正常。结论:4种大黄蒽醌类化合物对幽门螺杆菌均有较强的抑制生长作用。}

Four compounds of anthraquinone in Rheum officinale on Helicobacter pylori inhibition

OBJECTIVE: To study the effect of four anthraquinone extracts in Rheum officznale on Helicobacter pylori inhibition. METHODS: Four compounds of anthraquinone in Rheum officinale were extracted with gradient alkaline extraction. The susceptibilty of Helicobacter pylori inhibition was studied with the dilution method in test tube and the paper diffusion. RESULTS: The minimum inhibitory concentration and Helicobacter pylory inhibited annulus of anthraquinone extracts in Rheum officinale were emodin 0.40μg·ml<^-1(30 mm ) , rhein 0.60μg·ml^-1(28 mm),chrysophanol 0.78μg·ml(24 mm ) and aloe-emodin 0.85 μg·ml^-1(23 mm), respectively. Control sample--Rheum offzcinale extract was 17.24μg· ml^-1(15 mm).CONCLUSION: Four compounds of anthraquinone in kheunr Rheum officinale were effective in Helicobacter pylori inhibition.