Rapid Analysis of Glucose,Fructose, Sucrose,and Maltose in Honeys from Different Geographic Regions using Fourier Transform Infrared Spectroscopy and Multivariate Analysis |
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| Authors: | Jun Wang Michael M Kliks Soojin Jun Mel Jackson Qing X Li |
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| Affiliation: | Authors Wang and Li are with Dept. of Molecular Biosciences and Bioengineering and Author Jun is with Dept. of Human Nutrition, Food and Animal Science, Univ. of Hawaii of Manoa, Honolulu, HI, 96822, U.S.A. Author Kliks is with CTS Foundation, 3081G Paty Drive, Honolulu, HI, 96822, U.S.A. Author Jackson is with Product Development and Services, Hawaii Agriculture Research Center, Kunia, HI 96759, U.S.A. Direct inquiries to authors Jun and Li (E-mail: soojin@hawaii.edu, qingl@hawaii.edu). |
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| Abstract: | ABSTRACT: Quantitative analysis of glucose, fructose, sucrose, and maltose in different geographic origin honey samples in the world using the Fourier transform infrared (FTIR) spectroscopy and chemometrics such as partial least squares (PLS) and principal component regression was studied. The calibration series consisted of 45 standard mixtures, which were made up of glucose, fructose, sucrose, and maltose. There were distinct peak variations of all sugar mixtures in the spectral “fingerprint” region between 1500 and 800 cm?1. The calibration model was successfully validated using 7 synthetic blend sets of sugars. The PLS 2nd-derivative model showed the highest degree of prediction accuracy with a highest R2 value of 0.999. Along with the canonical variate analysis, the calibration model further validated by high-performance liquid chromatography measurements for commercial honey samples demonstrates that FTIR can qualitatively and quantitatively determine the presence of glucose, fructose, sucrose, and maltose in multiple regional honey samples. |
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| Keywords: | FTIR honey authentication quality sugars |
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