产纤维素酶黑曲霉LN0401液体发酵条件的分析

利用黑曲霉LN0401菌株液体发酵生产纤维素酶。分别考察培养基碳源、培养基氮源、培养时间、培养温度、培养起始pH及孢子悬液接种的体积分数等因素对菌株产纤维素酶性能的影响。确定了黑曲霉LN0401产纤维素酶的最优条件。即以质量分数为5%的稻草粉作为培养基碳源;质量分数为1%的蛋白胨作为培养基氮源;培养时间为3 d;培养温度为30℃;培养起始pH为6.0;孢子悬液接种的体积分数为6%~8%接入装有100 mL液体发酵培养基的250 mL三角瓶内,150 r/min振荡培养。此条件下黑曲霉LN0401的CMC酶活为195.6~198.5 U/mL,FPA酶活为27.7~30.4 U/mL。

Analysis of Submerged Fermentation Conditions of Aspergillus niger LN0401 to Produce Cellulase

The Aspergillus niger LN0401 was used to produce cellulase by submerged fermentation.The influence factors of cultural medium carbon source,cultural medium nitrogen source,cultural time,cultural temperature,initial pH,and inoculation volumic fraction of spore suspending liquid on produced cellulose ability of the stain were studied.The optimum conditions of producing cellulose are as follows.The carbon source of culture medium is straw powder,and its mass fraction is 5%.The nitrogen source of culture medium is peptone,and its mass fraction is 1%.The cultural time is 3 d.The cultural temperature is 30 ℃.The initial pH is 6.0.The 6%～8% inoculation volumic fraction of spore suspending liquid is added to 250 mL conical flask with 100 mL submerged culture medium.Shaking table revolution is 150 r/min.Under these conditions,the Aspergillus niger LN0401 CMC enzyme activity reaches to 195.6～198.5 U/mL,and FPA enzyme activity reaches to 27.7～30.4 U/mL.