| 罗汉果果实、茎叶总黄酮的含量测定 |
| |
| 引用本文： | 饶荣,杨若婧,邓媛媛,何枭宇,叶晓川,刘焱文.罗汉果果实、茎叶总黄酮的含量测定[J].中国药师,2012,15(1):7-9. |
| |
| 作者姓名： | 饶荣 杨若婧 邓媛媛 何枭宇 叶晓川 刘焱文 |
| |
| 作者单位： | 湖北中医药大学中药资源与中药复方省部共建教育部重点实验室 武汉430061 |
| |
| 基金项目： | 中国博士后科学基金资助项目(编号:2010041126) |
| |
| 摘 要： | 目的:建立罗汉果果实、茎叶提取物中总黄酮的含量测定方法.方法:利用分光光度法,以山奈苷为对照,氯化铝为显色剂,在检测波长为393 nm下测定其总黄酮含量.结果:回归方程为Y=0.389 6X +0.009 7(r =0.999 8),山奈苷在0.1～0.4 mg-ml-1范围内线性关系良好,其平均回收率为97.27％,RSD=2.33％(n=6).结论:此方法简便、灵敏、准确、可靠性强,可用于测定罗汉果果实、茎叶提取物中总黄酮的含量,为其质量控制提供了检测方法.
|
| 关 键 词： | 罗汉果 总黄酮 山奈苷 含量测定 |
| 收稿时间： | 9/6/2011 12:00:00 AM |
| 修稿时间： | 2011/10/18 0:00:00 |
Determination of Total Flavonoids in Siraitia grosvenori Swingle Fruit Extract and Vine Leaf Extract |
| |
| Rao Rong,Yang Ruojing,Deng Yuanyuan,He Xiaoyu,Ye Xiaochuan and Liu Yanwen.Determination of Total Flavonoids in Siraitia grosvenori Swingle Fruit Extract and Vine Leaf Extract[J].China Pharmacist,2012,15(1):7-9. |
| |
| Authors: | Rao Rong Yang Ruojing Deng Yuanyuan He Xiaoyu Ye Xiaochuan and Liu Yanwen |
| |
| Affiliation: | Hubei University of Traditional Chinese Medicine,Wuhan 430061,China;Hubei University of Traditional Chinese Medicine,Wuhan 430061,China;Hubei University of Traditional Chinese Medicine,Wuhan 430061,China;Hubei University of Traditional Chinese Medicine,Wuhan 430061,China;Hubei University of Traditional Chinese Medicine,Wuhan 430061,China;Hubei University of Traditional Chinese Medicine,Wuhan 430061,China |
| |
| Abstract: | Objective ： To establish a method for the determination of total flavonoids in Siraitia grosvenori Swingle fruit extract and vine leaf extract. Method： The content of flavonoids was determined at 393 nm by UV spectrophotometry with kaempferitrin as the standard control. Result： The regression equation was Y = 0. 389 6X ＋ 0. 009 7 （ r = 0. 9998 ） , kaempferitrin showed a good linear curve within the range of 0. 1-0.4 mg＇ml-1 ,and the recovery was 97.27% with RSD of 2. 33% （n =6）. Conclusion： The method is simple, quick, reproducible and reliability. It can be used for determining the content of total flavonoids in Siraitia grosvenori Swingle fruit ex- tract and vine leaf extract to provide the detection method for the quality control. |
| |
| Keywords: | Siraitia grosvenori Swingle Flavonoids Kaempferitrin Determination |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国药师》浏览原始摘要信息 |
|
点击此处可从《中国药师》下载全文 |
|
