| 饲养层细胞对胚胎干细胞的影响及其分离培养的影响因素 |
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| 引用本文： | 王颖,陈莉娜,张艳艳,关伟军.饲养层细胞对胚胎干细胞的影响及其分离培养的影响因素[J].中国畜牧兽医,2007,34(11):39-42. |
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| 作者姓名： | 王颖 陈莉娜 张艳艳 关伟军 |
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| 作者单位： | 1.中国农业科学院北京畜牧兽医研究所,北京 100094； 2.山西农业大学,太谷 030801；3.西北农林科技大学,杨凌 712100 |
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| 基金项目： | 国家自然科技资源平台建设项目（2005DKA21101）. |
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| 摘 要： | 胚胎干细胞及其相关研究是生命科学领域中最有活力、最有影响力、最有应用前景的研究方向之一，它与遗传工程，转基因技术等相结合具有重大的理论意义与实践价值。饲养层细胞在胚胎干细胞分离培养的过程中起着异常重要的作用。作者结合实践经验就饲养层细胞对胚胎干细胞的影响进行了概述，介绍了饲养层细胞的分类，并分析了饲养层细胞分离培养的影响因素。
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| 关 键 词： | 胚胎干细胞 饲养层细胞 分离培养 |
| 文章编号： | 1671-7236（2007）11-0039-03 |
| 修稿时间： | 2007-04-09 |
Effects on Embryonic Stem Cells and Influencing Factors of Isolation & Culture of Feeder Cells |
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| WANG Ying,CHEN Li-na,ZHANG Yan-yan,GUAN Wei-jun.Effects on Embryonic Stem Cells and Influencing Factors of Isolation & Culture of Feeder Cells[J].China Animal Husbandry & Veterinary Medicine,2007,34(11):39-42. |
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| Authors: | WANG Ying CHEN Li-na ZHANG Yan-yan GUAN Wei-jun |
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| Affiliation: | 1.Institute of Animal Science,Chinese Academy of Agriculture Science，Beijing 100094, China; 2.Shanxi Agricultural University, Taigu 030801, China; 3.Northwestern Sci tech University of Agriculture and Forestry,Yangling 712100,China |
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| Abstract: | Comprehensive research of stem cells which is one of the energiest, most impacting and applied foreground research directions in life science,when united with genetic engineering and transplantation may provide significance of theory and practice. Feeder cells have a great impact on isolation and culture of ES cells. Refering to plenty of information, the author combined with experiment experience explained the general impact of feeder cells on embryonic stem cells, introduced the class of feeder cells and discussed the influencing factores of isolation and culture. |
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| Keywords: | stem cells feeder cells isolation and culture |
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