THE IN VITRO EFFECTS OF STEROIDS, HUMAN CHORIONIC GONADOTROPIN AND CYANOKETONE ON GERMINAL BREAKDOWN OF STRIPED MULLET (MUGIL CEPHALUS L.)OOCYTES

The in vitro effects of steroids, human chorionic gonadotropin (HCG) and cyanoketone on germinal vesicle breakdown (GVBD) of striped mullet (Mugil cephalus L.) oocytes were investigated. All concentrations of HCG (5,10,50 I.U./ml), progesterone and pregnenolone at the highest concentrations(lug/ml) were moderately effective in inducing GVBD, whereas 17B-estrodiol, cortisol, testosterone, 11B-hydroxyandrostenedione and 11-ketotestosterone did not stimulate GVBD. 17a, 20Bdihydroxy-4-pregnen-3-one (17a, 20BdiOHprog) and deoxycorticosterone (DOC) were the most potent steroids in stimulating final oocyte maturation. The results indicate that C21 hydroxylated steroids are potent inducers of final maturation in mullet. Further, co-incubations with 17B-eslradiol, cortisol and testosterone did not alter the maturation-inducing effects of HCG or 17a,200diOHprog. Cyanoketone, a blocker of 3BHSD activity, was only partially effective in blocking GVBD induced by HCG. This suggests that A5 (pregnenolone derived) and